Show Order Info
SKU:MA1079
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:CH-19
Application:IHC-P, WB
Price: $200.00
Quantity:
Technical
Specs
Kit
Components
Gene
Information
Application
Data & Images
Reviews
Publications
FAQs

Overview

Product Name Anti-Pan-Cadherin Antibody (Monoclonal, CH-19)
SKU/Catalog Number MA1079
Description Mouse IgG monoclonal antibody for Pan-Cadherin, cadherin 1, type 1, E-cadherin (epithelial); cadherin 2, type 1, N-cadherin (neuronal); cadherin 3, type 1, P-cadherin (placental) (CDH1; CDH2; CDH3 ) detection. Tested with WB, IHC-P in Human;mouse;rat;rabbit;chicken;snake. No cross reactivity with other proteins.
Cite This Product Anti-Pan-Cadherin Antibody (Monoclonal, CH-19) CH-19 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1079)
Replacement Item This antibody may replace the following items: sc-9815|sc-9817|sc-9816|sc-11352 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Chicken, Human, Mouse, Rabbit, Rat, Snake
Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Synthetic peptide corresponding to the C-terminal amino acids of chicken N-Cadherin with an extra N-terminal lysine residue(24 amino acids) coupled to KLH.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number CH-19
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Cdh3
Protein Name Cadherin-1; Cadherin-2; Cadherin-3
Molecular Weight 90415 MW
Protein Function Cadherins are calcium-dependent cell adhesion proteins. .
Tissue Specificity CDH1: Non-neural epithelial tissues.
Sequence Similarities Contains 5 cadherin domains.
Subcellular Localization CDH1: Cell junction. Cell membrane; Single-passtype I membrane protein. Endosome. Golgi apparatus, trans-Golginetwork. Note: Colocalizes with DLGAP5 at sites of cell-cellcontact in intestinal epithelial cells. Anchored to actinmicrofilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calciuminflux, results in translocation from sites of cell-cell contactto the cytoplasm. Colocalizes with RAB11A endosomes during itstransport from the Golgi apparatus to the plasma membrane.
Cdh1: Cell junction. Cell membrane; Single-passtype I membrane protein. Endosome Golgi apparatus,trans-Golgi network Note: Colocalizes with DLGAP5 atsites of cell-cell contact in intestinal epithelial cells.Anchored to actin microfilaments through association with alpha-,beta- and gamma-catenin. Sequential proteolysis induced byapoptosis or calcium influx, results in translocation from sitesof cell-cell contact to the cytoplasm. Colocalizes with RAB11Aendosomes during its transport from the Golgi apparatus to theplasma membrane (By similarity).
Cdh1: Cell junction. Cell membrane; Single-passtype I membrane protein. Endosome Golgi apparatus,trans-Golgi network Note: Colocalizes with DLGAP5 atsites of cell-cell contact in intestinal epithelial cells.Anchored to actin microfilaments through association with alpha-,beta- and gamma-catenin. Sequential proteolysis induced byapoptosis or calcium influx, results in translocation from sitesof cell-cell contact to the cytoplasm. Colocalizes with RAB11Aendosomes during its transport from the Golgi apparatus to theplasma membrane (By similarity).
CDH2: Cell membrane; Single-pass type I membraneprotein.
Cdh2: Cell membrane; Single-pass type I membraneprotein.
Cdh2: Cell membrane
CDH3: Cell membrane; Single-pass type I membraneprotein.
Cdh3: Cell membrane; Single-pass type I membraneprotein.
Uniprot ID F1LMI3
Alternative Names Cadherin 3, type 1, P-cadherin (Placental) ;Protein Cdh3 ;Cdh3 ;rCG_51631 ;
Research Areas signal transduction|cytoskeleton / ecm|cell adhesion|cadherins|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Cadherin-1; Cadherin-2; Cadherin-3

Cadherins are calcium-dependent cell-cell adhesion molecules that mediate cell-cell binding in a homophilic manner. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherin expression is regulated spatially as well as temporally. Cadherins are though to play an important role in development and maintenance of tissues through selective cell-cell adhesion activity and may be involved also in the invasion and metastasis of malignant tumors. Cadherin regulates dendritic spine morphogenesis. A cadherin gene cluster is mapped to a region of chromosome 5 subject to frequent allelic loss in carcinoma.
Write a review for MA1079

Publications

Qiu J, Zhang C, Lv Y, Zhang Y, Zhu C, Wang X, Yao W. Neurochem Int. 2013 Aug;63(2):87-92. Doi: 10.1016/J.Neuint.2013.05.009. Epub 2013 May 28. Cdh1 Inhibits Reactive Astrocyte Proliferation After Oxygen-Glucose Deprivation And Reperfusion.

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to n-cadherin antibody, cdh3 antibody, p-cadherin antibody