Data & Images
|Product Name||Anti-PARP Antibody|
|Description||Rabbit IgG polyclonal antibody for Poly[ADP-ribose] polymerase 1(PARP1) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-PARP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2227)|
|Replacement Item||This antibody may replace the following items: sc-136208|sc-1561|sc-1562|sc-23461-R|sc-245198|sc-245199|sc-25780|sc-365315|sc-365315-X|sc-53643|sc-56196|sc-56197|sc-7150|sc-74469|sc-74469-X|sc-74470|sc-74470-X|sc-74808|sc-8007 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human PARP(78-91aa RWDDQQKVKKTAEA), identical to the related mouse and rat sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Poly [ADP-ribose] polymerase 1|
|Molecular Weight||113084 MW|
|Protein Function||Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP- ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. .|
|Sequence Similarities||Contains 1 BRCT domain.|
|Subcellular Localization||Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage.|
|Alternative Names||Poly [ADP-ribose] polymerase 1;PARP-1;22.214.171.124;ADP-ribosyltransferase diphtheria toxin-like 1;ARTD1;NAD(+) ADP-ribosyltransferase 1;ADPRT 1;Poly[ADP-ribose] synthase 1;PARP1;ADPRT, PPOL;|
|Research Areas|||epigenetics and nuclear signaling|chromatin modifying enzymes|adp-ribosylation||
Background for Poly [ADP-ribose] polymerase 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-PARP Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-PARP Antibody Images
Click the images to enlarge.
All lanes: Anti PARP1 (PA2227) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Predicted bind size: 113KD
Observed bind size: 113KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,