Anti-PARP/PARP1 Antibody Picoband™

PARP1 antibody

Boster Bio Anti-PARP/PARP1 Antibody Picoband™ catalog # PB9309. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 5 publication(s).

Product Info Summary

SKU: PB9309
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-PARP/PARP1 Antibody Picoband™

View all PARP1 Antibodies

SKU/Catalog Number

PB9309

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PARP/PARP1 Antibody Picoband™ catalog # PB9309. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PARP/PARP1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9309)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PARP recombinant protein (Position: Q670-R858). Human PARP shares 94% and 95% amino acid (aa) sequence identity with mouse and rat PARP, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9309 is reactive to PARP1 in Human, Mouse, Rat

Applications

PB9309 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

120 kDa

Calculated molecular weight

113.084kDa

Background of PARP1

Poly [ADP-ribose] polymerase 1 (PARP1), also known as ADPRT or PPOL is an enzyme that in humans is encoded by the PARP1 gene. PARP1 gene is mapped to 1q42.12. This gene encodes a chromatin-associated enzyme, poly (ADP-ribosyl)transferase, which modifies various nuclear proteins by poly (ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry , 2μg/ml, Human, Rat
Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For PARP1 (Source: Uniprot.org, NCBI)

Gene Name

PARP1

Full Name

Poly [ADP-ribose] polymerase 1

Weight

113.084kDa

Alternative Names

ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADPRT 1; ADPRT; ADPRTADP-ribosyltransferase NAD(+); EC 2.4.2; EC 2.4.2.30; NAD(+) ADP-ribosyltransferase 1; PARP apoptosis; PARP; PARP1; PARP-1; PARPADPRT1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; PPOL; PPOLpADPRT-1 PARP1 ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PPOL, pADPRT-1 poly(ADP-ribose) polymerase 1 poly [ADP-ribose] polymerase 1|ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)|ADP-ribosyltransferase NAD(+)|ADP-ribosyltransferase diphtheria toxin-like 1|DNA ADP-ribosyltransferase PARP1|NAD(+) ADP-ribosyltransferase 1|poly (ADP-ribose) polymerase family, member 1|poly(ADP-ribose) synthetase|poly(ADP-ribosyl)transferase|poly[ADP-ribose] synthase 1|protein poly-ADP-ribosyltransferase PARP1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PARP1, check out the PARP1 Infographic

PARP1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PARP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9309 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

CIDEC silencing attenuates diabetic nephropathy via inhibiting apoptosis and promoting autophagy

Down‐regulation of tyrosine aminotransferase at a frequently deleted region 16q22 contributes to the pathogenesis of hepatocellular carcinoma
Species: Human

Wang P,Wang C,Liu C. Antitumor effects of dioscin in A431 cells via adjusting ATM/p53-mediated cell apoptosis, DNA damage and migration. Oncol Lett. 2021 Jan;21(1):59.doi:10.3892/ol.2020.12321.Epub 2020 Nov 19.PMID:33281970;PMCID:PMC7709553.
Species: Human
PB9309 usage in article: APP:WB, SAMPLE:A431 CELLS, DILUTION:NA

Epigallocatechin-3-Gallate Ameliorates Glucocorticoid-Induced Osteoporosis of Rats in Vivo and in Vitro

Li Q, Li Z, Xu Xy, Guo Yl, Du F. Int J Mol Sci. 2010 Nov 16;11(11):4580-90. Doi: 10.3390/Ijms11114580. Neuroprotective Properties Of Picroside Ii In A Rat Model Of Focal Cerebral Ischemia.

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5 Customer Q&As for Anti-PARP/PARP1 Antibody Picoband™

Question

We purchased anti-PARP/PARP1 antibody for ICC on leukemic t-cell in the past. I am using mouse, and We want to use the antibody for IHC-P next. I was wanting to use examining leukemic t-cell as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?

Verified Customer

Verified customer

Asked: 2020-04-23

Answer

I looked at the website and datasheets of our anti-PARP/PARP1 antibody and it seems that PB9309 has been tested on mouse in both ICC and IHC-P. Thus PB9309 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-04-23

Question

My team were well pleased with the WB result of your anti-PARP/PARP1 antibody. However we have seen positive staining in fibroblast nucleus using this antibody. Is that expected? Could you tell me where is PARP1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-04-23

Answer

Based on literature, fibroblast does express PARP1. Generally PARP1 expresses in nucleus. Regarding which tissues have PARP1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, and Ovarian carcinoma, Pubmed ID: 17177976
Embryonic kidney, Pubmed ID: 17525332
Fibroblast, Pubmed ID: 3120710, 2824474, 2891139
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2020-04-23

Question

We have observed staining in human heart left ventricle. Do you have any suggestions? Is anti-PARP/PARP1 antibody supposed to stain heart left ventricle positively?

Verified Customer

Verified customer

Asked: 2019-06-13

Answer

Based on literature heart left ventricle does express PARP1. Based on Uniprot.org, PARP1 is expressed in heart left ventricle, fibroblast, brain, colon carcinoma ovarian carcinoma, embryonic kidney, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have PARP1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, and Ovarian carcinoma, Pubmed ID: 17177976
Embryonic kidney, Pubmed ID: 17525332
Fibroblast, Pubmed ID: 3120710, 2824474, 2891139
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2019-06-13

Question

We are currently using anti-PARP/PARP1 antibody PB9309 for mouse tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2018-08-08

Answer

The anti-PARP/PARP1 antibody (PB9309) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-08-08

Question

My lab would like using your anti-PARP/PARP1 antibody for detection of dna damage studies. Has this antibody been tested with western blotting on hela whole cell lysates? We would like to see some validation images before ordering.

E. Miller

Verified customer

Asked: 2015-05-22

Answer

Thanks for your inquiry. This PB9309 anti-PARP/PARP1 antibody is validated on human placenta tissue, hela whole cell lysates, hepg2 whole cell lysates, jurkat whole cell lysates, rat brain tissue, mouse intestine tissue, nih3t3 whole cell lysates. It is guaranteed to work for IF, IHC-P, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2015-05-22

Order DetailsPrice
PB9309

100μg

$370
PB9309-10ug

10μg sample (liquid)

$99
PB9309-Biotin

100 μg Biotin conjugated

$570
PB9309-Cy3

100 μg Cy3 conjugated

$570
PB9309-Dylight488

100 μg Dylight488 conjugated

$570
PB9309-Dylight550

100 μg Dylight550 conjugated

$570
PB9309-Dylight594

100 μg Dylight594 conjugated

$570
PB9309-FITC

100 μg FITC conjugated

$570
PB9309-HRP

100 μg HRP conjugated

$570
PB9309-APC

100 μg APC conjugated

$670
PB9309-PE

100 μg PE conjugated

$670
PB9309-carrier-free

Carrier Free

$370

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