|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-PBK Antibody|
|Description||Rabbit IgG polyclonal antibody for Lymphokine-activated killer T-cell-originated protein kinase(PBK) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-PBK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2305)|
|Replacement Item||This antibody may replace the following items: sc-390817|sc-136026|sc-293028|sc-390400|sc-366752|sc-292890|sc-390790|sc-390812|sc-390399|sc-393313|sc-15243-R from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human PBK(306-322aa KDRPSAAHIVEALETDV), different from the related mouse and rat sequences by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Lymphokine-activated killer T-cell-originated protein kinase|
|Molecular Weight||36085 MW|
|Protein Function||Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. .|
|Tissue Specificity||Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules. .|
|Sequence Similarities||Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.|
|Alternative Names||Lymphokine-activated killer T-cell-originated protein kinase;188.8.131.52;Cancer/testis antigen 84;CT84;MAPKK-like protein kinase;Nori-3;PDZ-binding kinase;Spermatogenesis-related protein kinase;SPK;T-LAK cell-originated protein kinase;PBK;TOPK;|
|Research Areas|||cell biology|cell cycle|cell cycle inhibitors|p53| cell biology|cell division|cytokinesis| epigenetics and nuclear signaling|dna / rna|dna damage & repair|dna damage response| signal transduction|protein phosphorylation|ser / thr kinases|mapk pathway| cancer|p53 pathway||
Background for Lymphokine-activated killer T-cell-originated protein kinase
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-PBK Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-PBK Antibody Images
Click the images to enlarge.
All lanes: Anti-PBK(PA2305) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: SKOV Whole Cell Lysate at 40ug
Lane 5: HEPG2 Whole Cell Lysate at 40ug
Lane 6: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 36KD
Observed bind size: 36KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,