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| SKU | MA1082 |
|---|---|
| Size | 100μg/vial |
| Reactivity | Human, Mouse, Rat |
| Clonality | Monoclonal |
| Clone Number | PCK-26 |
| Host | Mouse |
| Ig Isotype | Mouse IgG1 |
| Applications | IHC, WB |
Overview
| Product Name | Anti-PCK-26 Antibody (Monoclonal, PCK-26) |
|---|---|
| SKU/Catalog Number | MA1082 |
| Storage & Handling | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Size | 100μg/vial |
| Description | Mouse IgG monoclonal antibody for PCK-26 detection. Tested with WB, IHC-P, IHC-F in Human;mouse;rat. No cross reactivity with other proteins. |
| Cite This Product | Anti-PCK-26 Antibody (Monoclonal, PCK-26) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1082) |
| Host | Mouse |
| Contents/Buffer | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Immunogen | Cytokeratin from human epidermis. |
| Reactivity | Human, Mouse, Rat |
Assay Details
Assay Dilutions Overview
Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, By Heat
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, mouse, rat, -
Western blot, 0.5-1μg/ml, Human, mouse, rat
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, By Heat
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, mouse, rat, -
Western blot, 0.5-1μg/ml, Human, mouse, rat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).Images And Assay Conditions
Boster Kit Box
Target Info
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | Q10758 |
|---|---|
| Gene Name | Krt8 |
| Protein Name | Keratin, type II cytoskeletal 8 |
| Tissue Specificity | Expressed in cardiac and striated muscle. Expressed at Z-lines within the muscle fibers and at Z-line and M- line domains at costameres at the sarcolemmal membrane (at protein level). Observed in coagulating gland, bladder, salivary gland, kidney, spleen, thymus, lung and heart. Also observed in ventral prostate, seminal vesicle and liver where expression increases following castration. . |
| Alternative Names | Keratin, type II cytoskeletal 8;Cytokeratin endo A;Cytokeratin-8;CK-8;Keratin-8;K8;Type-II keratin Kb8;Krt8;Krt2-8; |
| Subcellular Localization | Cytoplasm . Nucleus, nucleoplasm . Nucleus matrix . |
| Molecular Weight | 54019 MW |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. . |
|---|---|
| Research Areas | Class I, Cytoskeleton, Cytoskeleton / Ecm, Intermediate Filaments, Keratin, Signal Transduction *You can search these to find other products in these research areas. |
| Background | Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin(mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. |
Other Recommended Resources
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Customer Q&As
Q: Is your western blot protocol provided from the website applicable for all your antibodies?
Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody?
Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.