| SKU: | MA1082 |
|---|---|
| Pack Size: | 100μg/vial |
| Validated Species: | Human, Mouse, Rat |
| Clonality: | Monoclonal |
| Clone Number: | PCK-26 |
| Host: | Mouse |
| Application: | IHC, WB |
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Q&As
Overview
| Product Name | Anti-PCK-26 Antibody (Monoclonal, PCK-26) |
|---|---|
| SKU/Catalog Number | MA1082 |
| Description | Mouse IgG monoclonal antibody for PCK-26 detection. Tested with WB, IHC-P, IHC-F in Human;mouse;rat. No cross reactivity with other proteins. |
| Cite This Product | Anti-PCK-26 Antibody (Monoclonal, PCK-26) PCK-26 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1082) |
| Replacement Item | This antibody may replace the following items: sc-6810|sc-6811|sc-12578|sc-12579 from Santa Cruz Biotechnology. |
| Host | Mouse |
| Validated Species | Human, Mouse, Rat |
| Application | IHC, WB *Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under Protein Target Info. |
| Recommended Detection Systems | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $50. Contact us for more information **Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018! |
| Immunogen | Cytokeratin from human epidermis. |
| Cross Reactivity | No cross reactivity with other proteins |
| Pack Size | 100μg/vial |
Properties
| Clonality | Monoclonal |
|---|---|
| Clone Number | PCK-26 |
| Form | Lyophilized |
| Contents | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Concentration | Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Storage | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Purification | Ascites |
| Isotype | Mouse IgG1 |
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
| Gene Name | Krt8 |
|---|---|
| Protein Name | Keratin, type II cytoskeletal 5 |
| Molecular Weight | 54019 MW |
| Protein Function | Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. . |
| Tissue Specificity | Expressed in cardiac and striated muscle. Expressed at Z-lines within the muscle fibers and at Z-line and M- line domains at costameres at the sarcolemmal membrane (at protein level). Observed in coagulating gland, bladder, salivary gland, kidney, spleen, thymus, lung and heart. Also observed in ventral prostate, seminal vesicle and liver where expression increases following castration. . |
| Sequence Similarities | Belongs to the intermediate filament family. |
| Subcellular Localization | Cytoplasm . Nucleus, nucleoplasm . Nucleus matrix . |
| Uniprot ID | Q10758 |
| Alternative Names | Keratin, type II cytoskeletal 8;Cytokeratin endo A;Cytokeratin-8;CK-8;Keratin-8;K8;Type-II keratin Kb8;Krt8;Krt2-8; |
| Research Areas | |signal transduction|cytoskeleton / ecm|cytoskeleton|intermediate filaments|class i|keratin| |
Background for Keratin, type II cytoskeletal 5
Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin(mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues.
Related Products
Goat Anti-Mouse IgG Secondary Antibody, HRP Conjugate (BA1054)
Price: FREE if purchase with Primary antibody
Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001)
Price: $100
SuperVision IHC Kit with anti-Mouse IgG (SV0001-1)
Price: $150
Customer Q&As
Q: Is your western blot protocol provided from the website applicable for all your antibodies?
Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody?
Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
