Anti-PCK-26 Antibody (Monoclonal, PCK-26)

Mouse IgG monoclonal antibody for PCK-26 detection. Tested with WB, IHC-P, IHC-F in Human;mouse;rat. No cross reactivity with other proteins.

SKU MA1082
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Clone Number PCK-26
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, WB

Overview

Product Name Anti-PCK-26 Antibody (Monoclonal, PCK-26)
See all Krt8 primary antibodies, ELISA kits and proteins
SKU/Catalog Number MA1082
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Monoclonal clone# G2 antibody for Cytokeratin 8/Krt8 detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. Cytokeratin 8/Krt8 information: Molecular Weight: 54019 MW; Subcellular Localization: Cytoplasm . Nucleus, nucleoplasm . Nucleus matrix ; Tissue Specificity: Expressed in cardiac and striated muscle. Expressed at Z-lines within the muscle fibers and at Z-line and M- line domains at costameres at the sarcolemmal membrane (at protein level). Observed in coagulating gland, bladder, salivary gland, kidney, spleen, thymus, lung and heart. Also observed in ventral prostate, seminal vesicle and liver where expression increases following castration.
Cite This Product Anti-PCK-26 Antibody (Monoclonal, PCK-26) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1082)
Specificity Anti-PCK-26 Antibody (Monoclonal, PCK-26) (MA1082) reacts with Human, Mouse, Rat Krt8, in native form and recombinant. Superfamily members of Krt8 are not reactive to MA1082.
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form Lyophilized
Reconstitution Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunogen Cytokeratin from human epidermis.
Reactivity Human, Mouse, Rat

Assay Details

Our Boster Quality Guarantee for Anti-PCK-26 Antibody (Monoclonal, PCK-26) covers its use in the following applications.

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, By Heat
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, mouse, rat, -
Western blot, 0.5-1μg/ml, Human, mouse, rat

Boster's Compatible Products

The following reagents are used to generate the images below for Anti-PCK-26 Antibody (Monoclonal, PCK-26) (MA1082).

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Images And Assay Conditions

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Boster Kit Box

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q10758
Gene Name Krt8
Protein Name Keratin, type II cytoskeletal 8
Tissue Specificity Expressed in cardiac and striated muscle. Expressed at Z-lines within the muscle fibers and at Z-line and M- line domains at costameres at the sarcolemmal membrane (at protein level). Observed in coagulating gland, bladder, salivary gland, kidney, spleen, thymus, lung and heart. Also observed in ventral prostate, seminal vesicle and liver where expression increases following castration. .
Alternative Names Keratin, type II cytoskeletal 8;Cytokeratin endo A;Cytokeratin-8;CK-8;Keratin-8;K8;Type-II keratin Kb8;Krt8;Krt2-8;
Subcellular Localization Cytoplasm . Nucleus, nucleoplasm . Nucleus matrix .
Molecular Weight 54019 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. .
Research Areas Human, Mouse, Rat

*You can search these to find other products in these research areas.
Background Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin(mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues.

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Publishing with Anti-PCK-26 Antibody (Monoclonal, PCK-26) (MA1082)? Please let us know so we can cite the reference in this product datasheet. Email us at [email protected]

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MA1082
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

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Customer Q&As

  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
  • Q: Can you provide monoclonal antibodies carrier free?
    A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to [email protected]
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