Anti-PCK-26 Antibody (Monoclonal, PCK-26)

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Datasheet

Protocols

COA

MSDS

SKU	MA1082
Size	100μg/vial
Reactivity	Human, Mouse, Rat
Clonality	Monoclonal
Clone Number	PCK-26
Host	Mouse
Ig Isotype	Mouse IgG1
Applications	IHC, WB

• Overview
• Assay Details
• Target Info
• Order

Overview

Product Name	Anti-PCK-26 Antibody (Monoclonal, PCK-26)
SKU/Catalog Number	MA1082
Storage & Handling	At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size	100μg/vial
Description	Mouse IgG monoclonal antibody for PCK-26 detection. Tested with WB, IHC-P, IHC-F in Human;mouse;rat. No cross reactivity with other proteins.
Cite This Product	Anti-PCK-26 Antibody (Monoclonal, PCK-26) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1082)
Host	Mouse
Contents/Buffer	Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form	Lyophilized
Immunogen	Cytokeratin from human epidermis.
Reactivity	Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, By Heat
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, mouse, rat, -
Western blot, 0.5-1μg/ml, Human, mouse, rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Images And Assay Conditions

Boster Kit Box

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id	Q10758
Gene Name	Krt8
Protein Name	Keratin, type II cytoskeletal 8
Tissue Specificity	Expressed in cardiac and striated muscle. Expressed at Z-lines within the muscle fibers and at Z-line and M- line domains at costameres at the sarcolemmal membrane (at protein level). Observed in coagulating gland, bladder, salivary gland, kidney, spleen, thymus, lung and heart. Also observed in ventral prostate, seminal vesicle and liver where expression increases following castration. .
Alternative Names	Keratin, type II cytoskeletal 8;Cytokeratin endo A;Cytokeratin-8;CK-8;Keratin-8;K8;Type-II keratin Kb8;Krt8;Krt2-8;
Subcellular Localization	Cytoplasm . Nucleus, nucleoplasm . Nucleus matrix .
Molecular Weight	54019 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function	Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. .
Research Areas	Human, Mouse, Rat *You can search these to find other products in these research areas.
Background	Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin(mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues.

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Monoclonal clone# G2 antibody for Cytokeratin 8/Krt8 detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. Cytokeratin 8/Krt8 information: Molecular Weight: 54019 MW; Subcellular Localization: Cytoplasm . Nucleus, nucleoplasm . Nucleus matrix ; Tissue Specificity: Expressed in cardiac and striated muscle. Expressed at Z-lines within the muscle fibers and at Z-line and M- line domains at costameres at the sarcolemmal membrane (at protein level). Observed in coagulating gland, bladder, salivary gland, kidney, spleen, thymus, lung and heart. Also observed in ventral prostate, seminal vesicle and liver where expression increases following castration.

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MA1082

Promotion :

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)

$280.00

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Troubleshooting

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Customer Q&As

• Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
  A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
• Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
  A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
• Q: Can you provide monoclonal antibodies carrier free?
  A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to support@bosterbio.com.