Anti-PCK-26 Krt8 Antibody (Monoclonal, PCK-26)

Product Name

Anti-PCK-26 Krt8 Antibody (Monoclonal, PCK-26)

View all Krt8 Antibodies

SKU/Catalog Number

MA1082

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PCK-26 Krt8 Antibody (Monoclonal, PCK-26) catalog # MA1082. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PCK-26 Krt8 Antibody (Monoclonal, PCK-26) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1082)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

PCK-26

Isotype

Mouse IgG1

Immunogen

Cytokeratin from human epidermis.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

MA1082 is reactive to Krt8 in Human, Mouse

Applications

MA1082 is guaranteed for IF, IHC, WB Boster Guarantee

Background of Krt8

Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.5-1μg/ml, Human, mouse
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, By Heat
Immunofluorescence, 1-2μg/ml, Human, -

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Pan cytokeratin using anti-Pan cytokeratin antibody (MA1082).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human NCL-H460 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: mouse skin tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Pan cytokeratin antigen affinity purified monoclonal antibody (Catalog # MA1082) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Pan cytokeratin at approximately 54 kDa. The expected band size for Pan cytokeratin is at 54 kDa.

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Figure 2. IHC analysis of Pan cytokeratin using anti-Pan cytokeratin antibody (MA1082).
Pan cytokeratin was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml mouse anti-Pan cytokeratin Antibody (MA1082) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.

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Figure 3. IF analysis of Pan cytokeratin using anti-Pan cytokeratin antibody (MA1082).
Pan cytokeratin was detected in a paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/mL mouse anti-Pan cytokeratin Antibody (MA1082) overnight at 4°C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody at 1: 500 and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For Krt8 (Source: Uniprot.org, NCBI)

Alternative Names

CARD2; CK8; CK-8; CYK8; Cytokeratin 8; Cytokeratin-8; EndoA; K2C8; K8; keratin 8; keratin, type II cytoskeletal 8; keratin-8; KO; KRT8; Type-II keratin Kb8 Krt8|CYKER, Krt2-8|keratin 8|keratin, type II cytoskeletal 8|CK-8|K8|cytokeratin endo A|cytokeratin-8|keratin 8, type II|keratin complex 2, basic, gene 8|type-II keratin Kb8

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