|Application:||IHC-P, IHC-F, WB|
Data & Images
|Product Name||Anti-PCK-26 Antibody (Monoclonal, PCK-26)|
|Description||Mouse IgG monoclonal antibody for PCK-26 detection. Tested with WB, IHC-P, IHC-F in Human;mouse;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-PCK-26 Antibody (Monoclonal, PCK-26) PCK-26 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1082)|
|Replacement Item||This antibody may replace the following items: sc-6810|sc-6811|sc-12578|sc-12579 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
|Application||IHC-P, IHC-F, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Cytokeratin from human epidermis.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Keratin, type II cytoskeletal 5|
|Molecular Weight||54019 MW|
|Protein Function||Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. .|
|Tissue Specificity||Expressed in cardiac and striated muscle. Expressed at Z-lines within the muscle fibers and at Z-line and M- line domains at costameres at the sarcolemmal membrane (at protein level). Observed in coagulating gland, bladder, salivary gland, kidney, spleen, thymus, lung and heart. Also observed in ventral prostate, seminal vesicle and liver where expression increases following castration. .|
|Sequence Similarities||Belongs to the intermediate filament family.|
|Subcellular Localization||Cytoplasm . Nucleus, nucleoplasm . Nucleus matrix .|
|Alternative Names||Keratin, type II cytoskeletal 8;Cytokeratin endo A;Cytokeratin-8;CK-8;Keratin-8;K8;Type-II keratin Kb8;Krt8;Krt2-8;|
|Research Areas|||signal transduction|cytoskeleton / ecm|cytoskeleton|intermediate filaments|class i|keratin||
Background for Keratin, type II cytoskeletal 5
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-PCK-26 Antibody (Monoclonal, PCK-26) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, By Heat
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, mouse, rat, -
Western blot, 0.5-1μg/ml, Human, mouse, rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.