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SKU:MA1081
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2
Application:IHC-P, IHC-F, WB
Price: $200.00
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Overview

Product Name Anti-PCK Antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2)
SKU/Catalog Number MA1081
Description Mouse IgG monoclonal antibody for PCK detection. Tested with WB, IHC-P, IHC-F in Human;rat. No cross reactivity with other proteins.
Cite This Product Anti-PCK Antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1081)
Host Mouse
Isotype Mouse IgG2a/ IgG1
Validated Species Human, Rat
Application IHC-P, IHC-F, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen mixture of several monoclonal cytokeratin clones.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG2a/ IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name KRT1
Protein Name Keratin, type II cytoskeletal 1
Molecular Weight 66039 MW
Protein Function May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK. .
Tissue Specificity The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis.
Sequence Similarities Belongs to the intermediate filament family.
Subcellular Localization Cell membrane . Located on plasma membrane of neuroblastoma NMB7 cells.
Uniprot ID P04264
Alternative Names Keratin, type II cytoskeletal 1;67 kDa cytokeratin;Cytokeratin-1;CK-1;Hair alpha protein;Keratin-1;K1;Type-II keratin Kb1;KRT1;KRTA;
Research Areas |signal transduction|cytoskeleton / ecm|cytoskeleton|intermediate filaments|class i|cytokeratins| signal transduction|microtubules|cytokeratin|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Keratin, type II cytoskeletal 1

Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin(mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues.

Anti-PCK Antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) Images

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Anti-PCK Antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2)
Anti-PCK antibody (monoclonal), MA1081, IHC(P)
IHC(P): Human Placenta Tissue
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Publications

Huang, D., Wu, J., Su, X., Yan, H., & Sun, Z. (2017). Effects of low dose of bisphenol A on the proliferation and mechanism of primary cultured prostate epithelial cells in rodents. Oncology Letters. doi: 10.3892/ol.2017.6469
Bai J, Liu H, Li D, Cui L, Wu X. Plos One. 2012;7(11):E49673. Doi: 10.1371/Journal.Pone.0049673. Epub 2012 Nov 20. Duodenum Clamping Trauma Induces Significant Postoperative Intraperitoneal Adhesions On A Rat Model.

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.