|Application:||IHC-P, IHC-F, WB|
Data & Images
|Product Name||Anti-PCK Antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2)|
|Description||Mouse IgG monoclonal antibody for PCK detection. Tested with WB, IHC-P, IHC-F in Human;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-PCK Antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1081)|
|Isotype||Mouse IgG2a/ IgG1|
|Validated Species||Human, Rat|
|Application||IHC-P, IHC-F, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||mixture of several monoclonal cytokeratin clones.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Isotype||Mouse IgG2a/ IgG1|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Keratin, type II cytoskeletal 1|
|Molecular Weight||66039 MW|
|Protein Function||May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK. .|
|Tissue Specificity||The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis.|
|Sequence Similarities||Belongs to the intermediate filament family.|
|Subcellular Localization||Cell membrane . Located on plasma membrane of neuroblastoma NMB7 cells.|
|Alternative Names||Keratin, type II cytoskeletal 1;67 kDa cytokeratin;Cytokeratin-1;CK-1;Hair alpha protein;Keratin-1;K1;Type-II keratin Kb1;KRT1;KRTA;|
|Research Areas|||signal transduction|cytoskeleton / ecm|cytoskeleton|intermediate filaments|class i|cytokeratins| signal transduction|microtubules|cytokeratin||
Background for Keratin, type II cytoskeletal 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-PCK Antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.1-0.5μg/ml, Human, rat, By Heat
Immunohistochemistry(Frozen Section), 0.1-0.5μg/ml, Human, rat, -
Western blot, 1-2μg/ml, Human, rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-PCK Antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) Images
Click the images to enlarge.
IHC(P): Human Placenta Tissue