Anti-PDGFR alpha Rabbit Monoclonal Antibody

PDGFR alpha antibody

Boster Bio Anti-PDGFR alpha Rabbit Monoclonal Antibody catalog # M00366. Tested in WB application. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: M00366
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-PDGFR alpha Rabbit Monoclonal Antibody

View all PDGFR alpha Antibodies

SKU/Catalog Number

M00366

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-PDGFR alpha Rabbit Monoclonal Antibody catalog # M00366. Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PDGFR alpha Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00366)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AAOE-16

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human PDGFR alpha

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00366 is reactive to PDGFRA in Human, Mouse, Rat

Applications

M00366 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

150 kDa

Calculated molecular weight

122.67kDa

Background of PDGFR alpha

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For PDGFRA (Source: Uniprot.org, NCBI)

Gene Name

PDGFRA

Full Name

Platelet-derived growth factor receptor alpha

Weight

122.67kDa

Superfamily

protein kinase superfamily

Alternative Names

alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGF R alpha; PDGFR alpha; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA PDGFRA CD140A, PDGFR-2, PDGFR2 platelet derived growth factor receptor alpha platelet-derived growth factor receptor alpha|CD140 antigen-like family member A|CD140a antigen|PDGF-R-alpha|alpha-type platelet-derived growth factor receptor|platelet-derived growth factor receptor 2|platelet-derived growth factor receptor, alpha polypeptide

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PDGFRA, check out the PDGFRA Infographic

PDGFRA infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PDGFRA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00366 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Effect of maternal feed restriction in dairy goats at different stages of gestation on skeletal muscle development and energy metabolism of kids at the time of births

PDGFR%u03B1 regulated by miR-34a and FoxO1 promotes adipogenesis in porcine intramuscular preadipocytes through Erk signaling pathway

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4 Customer Q&As for Anti-PDGFR alpha Rabbit Monoclonal Antibody

Question

Does M00366 require dry ice during shipping?

Verified customer

Asked: 2021-10-20

Answer

We ship the Anti-PDGFR Alpha Rabbit Monoclonal Antibody (M00366) with gel ice.

Boster Scientific Support

Answered: 2021-10-21

Question

Our lab were happy with the WB result of your anti-PDGFR alpha Rabbit Monoclonal antibody. However we have seen positive staining in placenta cell membrane using this antibody. Is that expected? Could you tell me where is PDGFRA supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-08-26

Answer

According to literature, placenta does express PDGFRA. Generally PDGFRA expresses in cell membrane. Regarding which tissues have PDGFRA expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 8586421
Brain, Pubmed ID: 2536956
Colon tumor, Pubmed ID: 7896447
Eosinophil, Pubmed ID: 12808148
Foreskin, Pubmed ID: 2544881
Lung, and Trachea, Pubmed ID: 14702039
Placenta, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-08-26

Question

We are currently using anti-PDGFR alpha Rabbit Monoclonal antibody M00366 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?

J. Parker

Verified customer

Asked: 2017-11-03

Answer

The anti-PDGFR alpha Rabbit Monoclonal antibody (M00366) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-11-03

Question

We have observed staining in human blood. Do you have any suggestions? Is anti-PDGFR alpha Rabbit Monoclonal antibody supposed to stain blood positively?

J. Jones

Verified customer

Asked: 2015-03-30

Answer

Based on literature blood does express PDGFRA. Based on Uniprot.org, PDGFRA is expressed in endometrium, foreskin, brain, blood, lung trachea, placenta, eosinophil, colon tumor, among other tissues. Regarding which tissues have PDGFRA expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 8586421
Brain, Pubmed ID: 2536956
Colon tumor, Pubmed ID: 7896447
Eosinophil, Pubmed ID: 12808148
Foreskin, Pubmed ID: 2544881
Lung, and Trachea, Pubmed ID: 14702039
Placenta, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2015-03-30

Order DetailsPrice
M00366

100μL

$370
M00366-30ul

30μL sample

$99
M00366-carrier-free

Carrier Free

$370

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