Data & Images
|Product Name||Anti-Perforin Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Perforin-1(PRF1) detection. Tested with WB in Human.|
|Cite This Product||Anti-Perforin Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9155)|
|Replacement Item||This antibody may replace the following items: sc-134187|sc-136994|sc-22274|sc-33655|sc-368396|sc-373943|sc-374346|sc-71877|sc-7417|sc-7418|sc-87523|sc-87524|sc-9105 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human Perforin recombinant protein (Position: E175-W555). Human Perforin shares 68% amino acid (aa) sequence identity with both mouse and rat Perforin.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||61377 MW|
|Protein Function||Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. .|
|Sequence Similarities||Belongs to the complement C6/C7/C8/C9 family.|
|Subcellular Localization||Cytoplasmic granule lumen. Secreted. Cell membrane; Multi-pass membrane protein. Endosome lumen. Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes.|
|Alternative Names||Perforin-1;P1;Cytolysin;Lymphocyte pore-forming protein;PFP;PRF1;PFP;|
|Research Areas|||immunology|adaptive immunity|t cells|cytotoxic cells| immunology|innate immunity|complement| kits/ lysates/ other|elisa kits|granzyme and perforins elisa kits||
Background for Perforin-1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Perforin Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Perforin Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti Perforin (PB9155) at 0.5ug/ml
WB: Recombinant Human Perforin Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
All lanes: Anti Perforin (PB9155) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Lane 3: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 61KD
Observed bind size: 61KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,