Product Info Summary
SKU: | M01378 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Periostin Monoclonal Antibody
View all Periostin/OSF-2 Antibodies
SKU/Catalog Number
M01378
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Periostin Monoclonal Antibody catalog # M01378. Tested in WB, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Periostin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01378)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABIF-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Periostin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M01378 is reactive to POSTN in Human
Applications
M01378 is guaranteed for Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
37 kDa
Calculated molecular weight
93.314kDa
Background of Periostin/OSF-2
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Periostin expression in 293T cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For POSTN (Source: Uniprot.org, NCBI)
Gene Name
POSTN
Full Name
Periostin
Weight
93.314kDa
Alternative Names
Fasciclin I-like; MGC119510; MGC119511; OSF2; OSF-2; OSF-2osteoblast specific factor 2 (fasciclin I-like); OSF2periodontal ligament-specific periostin; Osteoblast-specific factor 2; PDLPOSTN; periostin isoform thy2; periostin isoform thy4; periostin isoform thy6; periostin isoform thy8; Periostin; periostin, osteoblast specific factor; PNRP11-412K4.1; POSTN; TRIF52 POSTN OSF-2, OSF2, PDLPOSTN, PN periostin periostin|osteoblast specific factor 2 (fasciclin I-like)|periodontal ligament-specific periostin|periostin, osteoblast specific factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POSTN, check out the POSTN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POSTN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Periostin Monoclonal Antibody (M01378)
Hello CJ!
M01378 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Musashi-1 Enhances Glioblastoma Migration by Promoting ICAM1 Translation
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Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
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17 Customer Q&As for Anti-Periostin Monoclonal Antibody
Question
I was wanting to use your anti-Periostin Monoclonal antibody for WB for human pubmed:8363580} rc placenta eco:0000269 on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human pubmed:8363580} rc placenta {eco:0000269 identification?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
As indicated on the product datasheet, M01378 anti-Periostin Monoclonal antibody has been tested for Flow Cytometry, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human pubmed:8363580} rc placenta {eco:0000269 in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-23
Question
Will anti-Periostin Monoclonal antibody M01378 work for WB with pubmed:8363580} rc placenta {eco:0000269?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
According to the expression profile of pubmed:8363580} rc placenta eco:0000269, POSTN is highly expressed in pubmed:8363580 rc placenta eco:0000269. So, it is likely that anti-Periostin Monoclonal antibody M01378 will work for WB with pubmed:8363580 rc placenta {eco:0000269.
Boster Scientific Support
Answered: 2020-02-12
Question
We are interested in to test anti-Periostin Monoclonal antibody M01378 on human pubmed:8363580} rc placenta {eco:0000269 for research purposes, then I may be interested in using anti-Periostin Monoclonal antibody M01378 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
The products we sell, including anti-Periostin Monoclonal antibody M01378, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-05
Question
Do you have a BSA free version of anti-Periostin Monoclonal antibody M01378 available?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Periostin Monoclonal antibody M01378 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-04
Question
See below the WB image, lot number and protocol we used for pubmed:8363580} rc placenta {eco:0000269 using anti-Periostin Monoclonal antibody M01378. Please let me know if you require anything else.
R. Rodriguez
Verified customer
Asked: 2019-12-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-25
Question
What is the sample target on Western blot analysis in your product image for M01378?
Verified customer
Asked: 2019-12-09
Answer
Western blot analysis of the Anti-Periostin Monoclonal Antibody (M01378) expression in 293T cell lysate.
Boster Scientific Support
Answered: 2019-12-10
Question
Can you help my question with product M01378, anti-Periostin Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01378 anti-Periostin Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-27
Question
We have observed staining in human pubmed:8363580}. What should we do? Is anti-Periostin Monoclonal antibody supposed to stain pubmed:8363580} positively?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
Based on literature pubmed:8363580} does express POSTN. Based on Uniprot.org, POSTN is expressed in periodontal ligament, osteosarcoma eco:0000269, pubmed:8363580
rc placenta eco:0000269, pubmed:8363580, plasma, liver, among other tissues. Regarding which tissues have POSTN expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218, 24275569
Osteosarcoma, and Placenta, Pubmed ID: 8363580
Periodontal ligament, Pubmed ID: 15057823
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2019-05-24
Question
We have tried in the past anti-Periostin Monoclonal antibody for Flow Cytometry on pubmed:8363580} rc placenta eco:0000269 a few years ago. I am using human, and I plan to use the antibody for WB next. you antibody examining pubmed:8363580} rc placenta eco:0000269 as well as osteosarcoma eco:0000269 in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2018-08-24
Answer
I have checked the website and datasheets of our anti-Periostin Monoclonal antibody and it appears that M01378 has been tested on human in both Flow Cytometry and WB. Thus M01378 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-08-24
Question
We are currently using anti-Periostin Monoclonal antibody M01378 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-25
Answer
The anti-Periostin Monoclonal antibody (M01378) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-25
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pubmed:8363580} rc placenta {eco:0000269 using anti-Periostin Monoclonal antibody M01378. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-02-19
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-19
Question
Our lab were content with the WB result of your anti-Periostin Monoclonal antibody. However we have seen positive staining in liver golgi apparatus using this antibody. Is that expected? Could you tell me where is POSTN supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-05-18
Answer
Based on literature, liver does express POSTN. Generally POSTN expresses in golgi apparatus. Regarding which tissues have POSTN expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218, 24275569
Osteosarcoma, and Placenta, Pubmed ID: 8363580
Periodontal ligament, Pubmed ID: 15057823
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2017-05-18
Question
Does M01378 anti-Periostin Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
L. Anderson
Verified customer
Asked: 2017-05-05
Answer
It shows on the product datasheet, M01378 anti-Periostin Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-05-05
Question
Is a blocking peptide available for product anti-Periostin Monoclonal antibody (M01378)?
Z. Johnson
Verified customer
Asked: 2016-12-07
Answer
We do provide the blocking peptide for product anti-Periostin Monoclonal antibody (M01378). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-12-07
Question
I see that the anti-Periostin Monoclonal antibody M01378 works with WB, what is the protocol used to produce the result images on the product page?
A. Moore
Verified customer
Asked: 2016-11-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-11-24
Question
Is this M01378 anti-Periostin Monoclonal antibody reactive to the isotypes of POSTN?
N. Edwards
Verified customer
Asked: 2015-02-05
Answer
The immunogen of M01378 anti-Periostin Monoclonal antibody is A synthesized peptide derived from human Periostin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-02-05
Question
Would anti-Periostin Monoclonal antibody M01378 work on dog WB with plasma?
G. Krishna
Verified customer
Asked: 2013-09-06
Answer
Our lab technicians have not validated anti-Periostin Monoclonal antibody M01378 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Periostin Monoclonal antibody M01378 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog plasma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-09-06