Data & Images
|Product Name||Anti-PERK Antibody|
|Description||Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 2-alpha kinase 3(EIF2AK3) detection. Tested with WB, ICC in Human.|
|Cite This Product||Anti-PERK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2026)|
|Replacement Item||This antibody may replace the following items: sc-20425|sc-166297|sc-8095|sc-53010|sc-376235|sc-8094|sc-73022|sc-271669|sc-30110|sc-376235|sc-53010|sc-73022|sc-8094|sc-8095 from Santa Cruz Biotechnology.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human PERK(163-176aa QWDQDRESMETVPF).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Eukaryotic translation initiation factor 2-alpha kinase 3|
|Molecular Weight||125216 MW|
|Protein Function||Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function (By similarity). .|
|Tissue Specificity||Ubiquitous. A high level expression is seen in secretory tissues.|
|Sequence Similarities||Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.|
|Subcellular Localization||Endoplasmic reticulum membrane; Single-pass type I membrane protein.|
|Alternative Names||Eukaryotic translation initiation factor 2-alpha kinase 3;188.8.131.52;PRKR-like endoplasmic reticulum kinase;Pancreatic eIF2-alpha kinase;HsPEK;EIF2AK3;PEK, PERK;|
|Research Areas|||epigenetics and nuclear signaling|dna / rna|translation|regulation| epigenetics and nuclear signaling|rnai|eukaryotic initiation factors (eif's)| metabolism|types of disease|diabetes|obesity|metabolic disorders||
Background for Eukaryotic translation initiation factor 2-alpha kinase 3
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-PERK Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, -|
Immunocytochemistry , 0.5-1μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-PERK Antibody Images
Click the images to enlarge.
Recombinant Protein Detection Source: E.coli derived -recombinant Human EIF2AK3, 35.1KD (162aa tag+ D93-R246)
Lane 1: Recombinant Human EIF2AK3 Protein 10ng
Lane 2: Recombinant Human EIF2AK3 Protein 5ng
Lane 3: Recombinant Human EIF2AK3 Protein 2.5ng
ICC: A549 Cell
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,