Anti-Peroxin 3 PEX3 Antibody

PEX3 antibody

Boster Bio Anti-Peroxin 3 PEX3 Antibody catalog # A04166. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A04166
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-Peroxin 3 PEX3 Antibody

View all PEX3 Antibodies

SKU/Catalog Number

A04166

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Peroxin 3 PEX3 Antibody catalog # A04166. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Peroxin 3 PEX3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04166)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

5G7

Isotype

IgG

Immunogen

Synthesized peptide derived from the N-terminal region of human Peroxin 3. at AA rangle: 30-110

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04166 is reactive to PEX3 in Human, Mouse, Rat

Applications

A04166 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

42.14kDa

Background of PEX3

MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.

Pariser H, et al. Proc Natl Acad Sci U S A 2005 Aug 30; 102(35): 12407-12412
Nagumo H, et al. Biochem Biophys Res Commun 2001 Jan 26; 280(3): 605-609
Yamamoto H, et al. Arch Biochem Biophys 1998 Nov 15; 359(2): 151-159

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For PEX3 (Source: Uniprot.org, NCBI)

Gene Name

PEX3

Full Name

Peroxisomal biogenesis factor 3

Weight

42.14kDa

Superfamily

peroxin-3 family

Alternative Names

DKFZp686N14184; FLJ13531; peroxin-3; Peroxisomal assembly protein PEX3; peroxisomal biogenesis factor 3; transformation-related protein 18; TRG18 PEX3 PBD10A, PBD10B, TRG18 peroxisomal biogenesis factor 3 peroxisomal biogenesis factor 3|peroxin-3|peroxisomal assembly protein PEX3|transformation-related protein 18

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PEX3, check out the PEX3 Infographic

PEX3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PEX3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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13 Customer Q&As for Anti-Peroxin 3 PEX3 Antibody

Question

Would anti-Peroxin 3 antibody A04166 work for WB with adrenal tissue?

Verified Customer

Verified customer

Asked: 2020-03-18

Answer

According to the expression profile of adrenal tissue, PEX3 is highly expressed in adrenal tissue. So, it is likely that anti-Peroxin 3 antibody A04166 will work for WB with adrenal tissue.

Boster Scientific Support

Answered: 2020-03-18

Question

Will anti-Peroxin 3 antibody A04166 work on goat WB with adrenal tissue?

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

Our lab technicians have not tested anti-Peroxin 3 antibody A04166 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Peroxin 3 antibody A04166 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat adrenal tissue in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-31

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adrenal tissue using anti-Peroxin 3 antibody A04166. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-01-06

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-01-06

Question

Is this A04166 anti-Peroxin 3 antibody reactive to the isotypes of PEX3?

Verified Customer

Verified customer

Asked: 2019-12-09

Answer

The immunogen of A04166 anti-Peroxin 3 antibody is Synthesized peptide derived from the N-terminal region of human Peroxin 3. at AA rangle: 30-110. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-12-09

Question

I see that the anti-Peroxin 3 antibody A04166 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-08-09

Question

Is there a BSA free version of anti-Peroxin 3 antibody A04166 available?

A. Evans

Verified customer

Asked: 2019-07-23

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Peroxin 3 antibody A04166 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-07-23

Question

I was wanting to use your anti-Peroxin 3 antibody for WB for human adrenal tissue on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human adrenal tissue identification?

Verified Customer

Verified customer

Asked: 2019-04-30

Answer

As indicated on the product datasheet, A04166 anti-Peroxin 3 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human adrenal tissue in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-04-30

Question

Would A04166 anti-Peroxin 3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-03-14

Answer

You can see on the product datasheet, A04166 anti-Peroxin 3 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-03-14

Question

Can you help my question with product A04166, anti-Peroxin 3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-02-14

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04166 anti-Peroxin 3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-02-14

Question

See below the WB image, lot number and protocol we used for adrenal tissue using anti-Peroxin 3 antibody A04166. Please let me know if you require anything else.

T. Lewis

Verified customer

Asked: 2018-08-15

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-08-15

Question

I am interested in to test anti-Peroxin 3 antibody A04166 on human adrenal tissue for research purposes, then I may be interested in using anti-Peroxin 3 antibody A04166 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-04-11

Answer

The products we sell, including anti-Peroxin 3 antibody A04166, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-04-11

Question

Is a blocking peptide available for product anti-Peroxin 3 antibody (A04166)?

Verified Customer

Verified customer

Asked: 2017-11-14

Answer

We do provide the blocking peptide for product anti-Peroxin 3 antibody (A04166). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-11-14

Question

We are currently using anti-Peroxin 3 antibody A04166 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?

A. Zhao

Verified customer

Asked: 2016-09-15

Answer

The anti-Peroxin 3 antibody (A04166) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-09-15

Order DetailsPrice
A04166

100uL

$399

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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