|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-Peroxiredoxin 1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Peroxiredoxin-1(PRDX1) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-Peroxiredoxin 1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1772)|
|Replacement Item||This antibody may replace the following items: sc-137150|sc-137212|sc-137222|sc-23965|sc-23969|sc-23970|sc-271020|sc-271047|sc-33571|sc-33574|sc-373961|sc-373962|sc-59656|sc-59657|sc-7381 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Peroxiredoxin 1(185-199aa KPDVQKSKEYFSKQK), different from the related rat and mouse sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||22110 MW|
|Protein Function||Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation (By similarity). .|
|Sequence Similarities||Belongs to the AhpC/TSA family.|
|Subcellular Localization||Cytoplasm . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.|
|Alternative Names||Peroxiredoxin-1;184.108.40.206;Natural killer cell-enhancing factor A;NKEF-A;Proliferation-associated gene protein;PAG;Thioredoxin peroxidase 2;Thioredoxin-dependent peroxide reductase 2;PRDX1;PAGA, PAGB, TDPX2;|
|Research Areas|||cell biology|other antibodies|oxidative stress| cell biology| cardiovascular|atherosclerosis|vascular inflammation|inflammatory mediators| metabolism|pathways and processes|redox metabolism||
Background for Peroxiredoxin-1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Peroxiredoxin 1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Peroxiredoxin 1 Antibody Images
Click the images to enlarge.
All lanes: Anti Peroxiredoxin 1 (PA1772) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: 563T Whole Cell Lysate at 40ug
Lane 3: Rat Brain Tissue Lysate at 50ug
Predicted bind size: 22KD
Observed bind size: 22KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,