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SKU:PA1834
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-Peroxiredoxin 2 Antibody
Description Rabbit IgG polyclonal antibody for Peroxiredoxin-2(PRDX2) detection. Tested with WB in Human;Mouse;Rat.
Cite This Product Anti-Peroxiredoxin 2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1834)
Replacement Item This antibody may replace the following items: sc-137150|sc-137212|sc-137222|sc-23967|sc-23969|sc-23970|sc-271020|sc-271047|sc-33571|sc-33572|sc-33574|sc-373961|sc-373962 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Peroxiredoxin 2(181-198aa DTIKPNVDDSKEYFSKHN), identical to the related mouse and rat sequences.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name PRDX2
Protein Name Peroxiredoxin-2
Molecular Weight 21892 MW
Protein Function Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).
Sequence Similarities Belongs to the AhpC/TSA family.
Subcellular Localization Cytoplasm.
Uniprot ID P32119
Alternative Names Peroxiredoxin-2;1.11.1.15;Natural killer cell-enhancing factor B;NKEF-B;PRP;Thiol-specific antioxidant protein;TSA;Thioredoxin peroxidase 1;Thioredoxin-dependent peroxide reductase 1;PRDX2;NKEFB, TDPX1;
Research Areas |neuroscience|neurology process|neurodegenerative disease|alzheimer's disease| cell biology|other antibodies|oxidative stress| cancer|cancer metabolism|cellular metabolic process| metabolism|pathways and processes|redox metabolism|types of disease|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Peroxiredoxin-2

PRDX2(peroxiredoxin 2) also known as NKEFB, PRP, PRX2, PRXII or TPX1, is a protein that in humans is encoded by the PRDX2 gene. This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. The Prdx2 -/- mice were healthy in appearance and fertile. Choi et al.(2005) demonstrated that PRDX2 is a negative regulator of PDGF signaling. Prx II deficiency results in increased production of peroxide, enhanced activation of PDGF receptor and phospholipase C-gamma-1, and subsequently increased cell proliferation and migration in response to PDGF. PRX2 accounted for about half of the thioredoxin activity in parasite extracts, and PRX2 expression was increased in the presence of chloroquine, regardless of P. falciparum strain susceptibility to the drug. 

Anti-Peroxiredoxin 2 Antibody Images

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Anti-Peroxiredoxin 2 Antibody
Anti-Peroxiredoxin 2 antibody, PA1834, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: HELA Cell Lysate
Lane 4: JURKAT Cell Lysate
Lane 5: 293T Cell Lysate
Lane 6: A549 Cell Lysate
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Publications

You X, Zhang Y, Li L, Li Z, Li M, Li C, Zhu J, Peng H, Sun J. Mol Biol Rep. 2014 Jul;41(7):4351-60. Doi: 10.1007/S11033-014-3306-3. Epub 2014 Mar 4. Cloning And Molecular Characterization Of Phospholipase D (Pld) Delta Gene From Longan (Dimocarpus...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to prp antibody, peroxiredoxin-2 antibody, prx2 antibody, tsa antibody