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SKU:PA1836
Clonality:Polyclonal
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-Peroxiredoxin 3 Antibody
SKU/Catalog Number PA1836
Description Rabbit IgG polyclonal antibody for Thioredoxin-dependent peroxide reductase, mitochondrial(PRDX3) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-Peroxiredoxin 3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1836)
Replacement Item This antibody may replace the following items: sc-130336|sc-137150|sc-137212|sc-137222|sc-23969|sc-23970|sc-23973|sc-271020|sc-271047|sc-33574|sc-373961|sc-373962|sc-59661|sc-59663 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of mouse Peroxiredoxin 3(240-257aa TIKPSPTASKEYFEKVHQ), identical to the related rat sequence, and different from the related human sequence by three amino acids.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Prdx3
Protein Name Thioredoxin-dependent peroxide reductase, mitochondrial
Molecular Weight 28127 MW
Protein Function Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical- generating system.
Tissue Specificity Housekeeping-type gene preferentially expressed in murine erythroleukemia (MEL) cells.
Sequence Similarities Belongs to the AhpC/TSA family.
Subcellular Localization Mitochondrion .
Uniprot ID P20108
Alternative Names Thioredoxin-dependent peroxide reductase, mitochondrial;1.11.1.15;Antioxidant protein 1;AOP-1;PRX III;Perioredoxin-3;Protein MER5;Prdx3;Aop1, Mer5;
Research Areas |cell biology|apoptosis|mitochondrial| signal transduction|metabolism| cell biology|other antibodies|oxidative stress| cancer|invasion/microenvironment|cytochrome c|cancer metabolism|cellular metabolic process| metabolism|pathways and processes|mitochondrial metabolism|mitochondrial markers|redox metabolism|metabolism processes|types of disease|cell death|apoptotic markers|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Thioredoxin-dependent peroxide reductase, mitochondrial

PRDX3(peroxiredoxin 3) also known as AOP-1, MER5, SP-22 or PRX3, is localized exclusively in mitochondria. The deduced 256-amino acid human AOP1 protein shares 86% amino acid sequence similarity with mouse Aop1, and significant similarity with both the human proliferation-associated gene A product and the mouse stress-induced peritoneal macrophage protein Msp23. The PRDX3 gene is mapped on 10q26.11. Expression of PRDX3 is induced by MYC and is reduced in c-myc -/- cells. Chromatin immunoprecipitation analysis spanning the entire PRDX3 genomic sequence revealed that MYC binds preferentially to a 930-bp region surrounding exon 1. Results using mitochondria-specific fluorescent probes demonstrated that PRDX3 is essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells. These data provided evidence that PRDX3 is a MYC target gene that is required to maintain normal mitochondrial function.

Anti-Peroxiredoxin 3 Antibody Images

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Anti-Peroxiredoxin 3 Antibody
Anti-Peroxiredoxin 3 antibody, PA1836, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Anti-Peroxiredoxin 3 Antibody
Anti-Peroxiredoxin 3 antibody, PA1836, IHC(P)
IHC(P): Rat Intestine Tissue
Anti-Peroxiredoxin 3 Antibody
Anti-Peroxiredoxin 3 antibody, PA1836, IHC(P)
IHC(P): Rat Intestine Tissue
Anti-Peroxiredoxin 3 Antibody
Anti-Peroxiredoxin 3 antibody, PA1836, Western blotting
All lanes: Anti Peroxiredoxin 3 (PA1836) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Lung Tissue Lysate at 50ug
Lane 3: Rat Kidney Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Lane 6: 293T Whole Cell Lysate at 40ug
Lane 7: MCF-7 Whole Cell Lysate at 40ug
Lane 8: A549 Whole Cell Lysate at 40ug
Lane 9: U20S Whole Cell Lysate at 40ug
Predicted bind size: 28KD
Observed bind size: 28KD
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to peroxiredoxin 3 antibody, peroxiredoxin-3 antibody