Anti-Peroxiredoxin 5/PRDX5 Antibody

Peroxiredoxin 5 antibody

Boster Bio Anti-Peroxiredoxin 5/PRDX5 Antibody catalog # PA1838. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA1838
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Peroxiredoxin 5/PRDX5 Antibody

View all Peroxiredoxin 5 Antibodies

SKU/Catalog Number

PA1838

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Peroxiredoxin 5/PRDX5 Antibody catalog # PA1838. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Peroxiredoxin 5/PRDX5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1838)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Peroxiredoxin 5, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1838 is reactive to PRDX5 in Human, Mouse, Rat

Applications

PA1838 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

26 kDa

Calculated molecular weight

22.086kDa

Background of Peroxiredoxin 5

PRDX5 (peroxiredoxin 5) also known as AOEB166, ACR1,B166, MGC117264, MGC142283, MGC142285, PLP, PMP20, PRDX6, PRXV, SBBI10, is a member of the peroxiredoxin family and may play an antioxidant protective role in various tissues under nonpathologic conditions and during inflammatory processes. The PRDX5 gene is mapped on 11q13.1. PRDX5 displays mitochondrial presequence features and has 3 cysteines implicated in antioxidant activity and a C-terminal SQL peroxisomal targeting sequence. Northern blot analysis revealed ubiquitous expression of a 1.0-kb PRDX5 transcript in tissues and cell lines. Functional analysis showed that PRDX5 has antioxidant activity equivalent to that of CAT. While PRDX5 was localized to fibroblasts in normal tendon, it was localized to fibroblasts and endothelial cells in degenerative tendon. PRDX5 mRNA and protein levels increased at 12 hours, and the increase in PRDX5 expression correlated with reduced peroxide levels. PRDX5 plays a protective role against oxidative stress in human cartilage.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For PRDX5 (Source: Uniprot.org, NCBI)

Gene Name

PRDX5

Full Name

Peroxiredoxin-5, mitochondrial

Weight

22.086kDa

Superfamily

peroxiredoxin family

Alternative Names

ACR1; ACR1Peroxisomal antioxidant enzyme; Alu corepressor 1; Alu co-repressor 1; Antioxidant enzyme B166; AOEB166; AOEB166Peroxiredoxin V; AOPP; B166; EC 1.11.1.15; Liver tissue 2D-page spot 71B; MGC117264; MGC142283; MGC142285; Peroxiredoxin 5; peroxiredoxin-5, mitochondrial; PLPThioredoxin peroxidase PMP20; PMP20; PRDX5; PRDX6; PRXV; prx-V; SBBI10; Thioredoxin reductase; TPx type VI PRDX5 ACR1, AOEB166, B166, HEL-S-55, PLP, PMP20, PRDX6, PRXV, SBBI10, prx-V peroxiredoxin 5 peroxiredoxin-5, mitochondrial|Alu co-repressor 1|TPx type VI|antioxidant enzyme B166|epididymis secretory protein Li 55|liver tissue 2D-page spot 71B|peroxiredoxin V|peroxisomal antioxidant enzyme|thioredoxin peroxidase PMP20|thioredoxin reductase|thioredoxin-dependent peroxiredoxin 5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PRDX5, check out the PRDX5 Infographic

PRDX5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PRDX5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for PA1838

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-Peroxiredoxin 5/PRDX5 Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Peroxiredoxin 5/PRDX5 Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

4 Customer Q&As for Anti-Peroxiredoxin 5/PRDX5 Antibody

Question

Will PA1838 anti-Peroxiredoxin 5/PRDX5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

A. Anderson

Verified customer

Asked: 2020-01-21

Answer

As indicated on the product datasheet, PA1838 anti-Peroxiredoxin 5/PRDX5 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-21

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Peroxiredoxin 5/PRDX5 antibody PA1838. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-09-13

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-13

Question

We need to test anti-Peroxiredoxin 5/PRDX5 antibody PA1838 on mouse liver for research purposes, then I may be interested in using anti-Peroxiredoxin 5/PRDX5 antibody PA1838 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-01-19

Answer

The products we sell, including anti-Peroxiredoxin 5/PRDX5 antibody PA1838, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-01-19

Question

I see that the anti-Peroxiredoxin 5/PRDX5 antibody PA1838 works with IHC, what is the protocol used to produce the result images on the product page?

D. Williams

Verified customer

Asked: 2017-03-23

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-03-23

Order DetailsPrice
PA1838

100μg

$370
PA1838-10ug

10μg sample (liquid)

$99
PA1838-Biotin

100 μg Biotin conjugated

$570
PA1838-Cy3

100 μg Cy3 conjugated

$570
PA1838-Dylight488

100 μg Dylight488 conjugated

$570
PA1838-Dylight550

100 μg Dylight550 conjugated

$570
PA1838-Dylight594

100 μg Dylight594 conjugated

$570
PA1838-FITC

100 μg FITC conjugated

$570
PA1838-HRP

100 μg HRP conjugated

$570
PA1838-APC

100 μg APC conjugated

$670
PA1838-PE

100 μg PE conjugated

$670
PA1838-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
PA1838
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.