Anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal Antibody

CDC2/CDK1 antibody

Boster Bio Anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal Antibody catalog # P00209-1. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat. Cited in 4 publication(s).

Product Info Summary

SKU: P00209-1
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IP, IHC, WB

Product Name

Anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal Antibody

View all CDC2/CDK1 Antibodies

SKU/Catalog Number

P00209-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal Antibody catalog # P00209-1. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00209-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

DFH-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Phospho-Cdk1/2 (T14)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P00209-1 is reactive to CDK1 in Human, Mouse, Rat

Applications

P00209-1 is guaranteed for IP, IHC, WB Boster Guarantee

Observed Molecular Weight

100 kDa

Calculated molecular weight

34.095kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For CDK1 (Source: Uniprot.org, NCBI)

Gene Name

CDK1

Full Name

Cyclin-dependent kinase 1

Weight

34.095kDa

Superfamily

protein kinase superfamily

Alternative Names

CDC2; CDC28A; CDC2MGC111195; CDK1; cell cycle controller CDC2; Cell division control protein 2 homolog; Cell division protein kinase 1; cyclin-dependent kinase 1; DKFZp686L20222; EC 2.7.11.22; EC 2.7.11.23; G1 to S and G2 to M; p34 protein kinase; P34CDC2 CDK1 CDC2, CDC28A, P34CDC2 cyclin dependent kinase 1 cyclin-dependent kinase 1|cell cycle controller CDC2|cell division control protein 2 homolog|cell division cycle 2, G1 to S and G2 to M|cell division protein kinase 1|p34 protein kinase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CDK1, check out the CDK1 Infographic

CDK1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CDK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

P00209-1 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

A novel cell cycle blocker extracted from Stellera chamaejasme L. inhibits the proliferation of hepatocarcinoma cells

Human enterovirus 68 interferes with the host cell cycle to facilitate viral production

Coxsackievirus A6 induces cell cycle arrest in G0/G1 phase for viral production

Glycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in Rats

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5 Customer Q&As for Anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal Antibody

Question

We have been able to see staining in mouse lymphoblast. What should we do? Is anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal antibody supposed to stain lymphoblast positively?

Verified Customer

Verified customer

Asked: 2020-02-07

Answer

From what I have seen in literature lymphoblast does express CDK1. From what I have seen in Uniprot.org, CDK1 is expressed in intestine, mammary cancer, skin, lymphoblast, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CDK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoblast, Pubmed ID: 14654843
Mammary cancer, Pubmed ID: 9515786, 14702039
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-02-07

Question

We are currently using anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal antibody P00209-1 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-08

Answer

The anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal antibody (P00209-1) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-08

Question

We ordered your anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal antibody for WB on cervix carcinoma erythroleukemia last year. I am using rat, and I plan to use the antibody for IP next. Our lab want to know about examining cervix carcinoma erythroleukemia as well as cervix carcinoma in our next experiment. Could give a recommendation on which antibody would work the best for IP?

Verified Customer

Verified customer

Asked: 2019-09-18

Answer

I have checked the website and datasheets of our anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal antibody and I see that P00209-1 has been tested on rat in both WB and IP. Thus P00209-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-09-18

Question

Our lab were well pleased with the WB result of your anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal antibody. However we have been able to see positive staining in cervix carcinoma nucleus. cytoplasm. mitochondrion. using this antibody. Is that expected? Could you tell me where is CDK1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

From literature, cervix carcinoma does express CDK1. Generally CDK1 expresses in nucleus. cytoplasm. mitochondrion. Regarding which tissues have CDK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoblast, Pubmed ID: 14654843
Mammary cancer, Pubmed ID: 9515786, 14702039
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-05-21

Question

My question regards using your anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal antibody for tp53 regulates transcription of genes involved in g2 cell cycle arrest studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-01-29

Answer

Thank you for your inquiry. This P00209-1 anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IP, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-01-29

Order DetailsPrice
P00209-1

100μL

$370
P00209-1-30ul

30μL sample

$99
P00209-1-carrier-free

Carrier Free

$370

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