Anti-Phospho-Chk1 (S296) CHEK1 Rabbit Monoclonal Antibody

Chk1 antibody

Boster Bio Anti-Phospho-Chk1 (S296) CHEK1 Rabbit Monoclonal Antibody catalog # P01060. Tested in WB application. This antibody reacts with Human.

Product Info Summary

SKU: P01060
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-Phospho-Chk1 (S296) CHEK1 Rabbit Monoclonal Antibody

View all Chk1 Antibodies

SKU/Catalog Number

P01060

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Phospho-Chk1 (S296) CHEK1 Rabbit Monoclonal Antibody catalog # P01060. Tested in WB application. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-Chk1 (S296) CHEK1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01060)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

FEO-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Phospho-Chk1 (S296)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P01060 is reactive to CHEK1 in Human

Applications

P01060 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

80 kDa

Calculated molecular weight

54.434kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For CHEK1 (Source: Uniprot.org, NCBI)

Gene Name

CHEK1

Full Name

Serine/threonine-protein kinase Chk1

Weight

54.434kDa

Superfamily

protein kinase superfamily

Alternative Names

Checkpoint, S. pombe, homolog of, 1; CHEK1; CHK1 (checkpoint, S.pombe) homolog; CHK1 checkpoint homolog (S. pombe); Chk1; CHK1serine/threonine-protein kinase Chk1; EC 2.7.11; EC 2.7.11.1 CHEK1 CHK1 checkpoint kinase 1 serine/threonine-protein kinase Chk1|CHK1 checkpoint homolog|Checkpoint, S. pombe, homolog of, 1|Chk1-S|cell cycle checkpoint kinase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CHEK1, check out the CHEK1 Infographic

CHEK1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CHEK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-Phospho-Chk1 (S296) CHEK1 Rabbit Monoclonal Antibody

Question

We have observed staining in human brain testis. What should we do? Is anti-Phospho-Chk1 (S296) Rabbit Monoclonal antibody supposed to stain brain testis positively?

Verified Customer

Verified customer

Asked: 2020-04-30

Answer

From literature brain testis does express CHEK1. From Uniprot.org, CHEK1 is expressed in secondary oocyte, fetal thymus, brain testis, bone marrow muscle, embryonic kidney, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CHEK1 expression, here are a few articles citing expression in various tissues:
Bone marrow, and Muscle, Pubmed ID: 15489334
Brain, and Testis, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Fetal thymus, Pubmed ID: 22184239
Leukemic T-cell, Pubmed ID: 19690332

Boster Scientific Support

Answered: 2020-04-30

Question

We are currently using anti-Phospho-Chk1 (S296) Rabbit Monoclonal antibody P01060 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-30

Answer

The anti-Phospho-Chk1 (S296) Rabbit Monoclonal antibody (P01060) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-30

Question

My boss were content with the WB result of your anti-Phospho-Chk1 (S296) Rabbit Monoclonal antibody. However we have observed positive staining in leukemic t-cell nucleus. cytoplasm. cytoplasm using this antibody. Is that expected? Could you tell me where is CHEK1 supposed to be expressed?

G. Rodriguez

Verified customer

Asked: 2013-10-10

Answer

From literature, leukemic t-cell does express CHEK1. Generally CHEK1 expresses in nucleus. cytoplasm. cytoplasm, cytoskeleton,. Regarding which tissues have CHEK1 expression, here are a few articles citing expression in various tissues:
Bone marrow, and Muscle, Pubmed ID: 15489334
Brain, and Testis, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Fetal thymus, Pubmed ID: 22184239
Leukemic T-cell, Pubmed ID: 19690332

Boster Scientific Support

Answered: 2013-10-10

Order DetailsPrice
P01060

100μL

$370
P01060-30ul

30μL sample

$99
P01060-carrier-free

Carrier Free

$370

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