Anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) Rabbit Monoclonal Antibody

SKU P00104
Size 100ug/vial
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Clone Number BIH-13
Host Rabbit
Applications IP, WB

Overview

Product Name Anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) Rabbit Monoclonal Antibody
SKU/Catalog Number P00104
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Size 100ug/vial
Description Anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) Rabbit Monoclonal Antibody tested for IP, WB in Human, Mouse, Rat
Cite This Product Anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00104)
Host Rabbit
Contents/Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Immunogen A synthesized peptide derived from human Phospho-ERK1 (T202/Y204) + ERK2 (T185/Y187)
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
WB 1:500-1:2000
IP 1:50

Images And Assay Conditions

/P/0/P00104 MAPK3 primary antibodies WB testing 1.jpg

Western blot analysis of Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) expression in A431 cell lysate treated with EGF (P00104).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK3 monoclonal antibody (Catalog # P00104) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAPK3

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P27361
Gene Name MAPK3
Protein Name Mitogen-activated protein kinase 3
Alternative Names Mitogen-activated protein kinase 3;MAP kinase 3;MAPK 3;2.7.11.24;ERT2;Extracellular signal-regulated kinase 1;ERK-1;Insulin-stimulated MAP2 kinase;MAP kinase isoform p44;p44-MAPK;Microtubule-associated protein 2 kinase;p44-ERK1;MAPK3;ERK1, PRKM3;
Subcellular Localization Cytoplasm. Nucleus. Autophosphorylation at Thr-207 promotes nuclear localization.
Molecular Weight 43136 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. .
Research Areas Alzheimer'S Disease, Cytoplasmic, Mapk Pathway, Neurodegenerative Disease, Neurology Process, Neuroscience, Protein Phosphorylation, Ser / Thr Kinases, Signal Transduction, Signaling Pathways, Stem Cells, Tgf Beta

*You can search these to find other products in these research areas.

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Order Product (P00104)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
100ug $299
100ug+Free HRP Secondary BA1054 $299
100ug+Free Biotin Secondary BA1003 $299
30ug sample size $99

USD $299

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Publications

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Synthesis and biological evaluation of novel N, N%u2032-disubstituted urea and thiourea derivatives as potential anti-melanoma agents
Disulfiram inhibits TGF-?-induced epithelial-mesenchymal transition and stem-like features in breast cancer via ERK/NF-?B/Snail pathway
Fructus phyllanthi tannin fraction induces apoptosis and inhibits migration and invasion of human lung squamous carcinoma cells in vitro via MAPK/MMP pathways
Ma Hr, Wang J, Chen Yf, Chen H, Wang Ws, Aisa Ha. Int J Mol Med. 2014 Jun;33(6):1627-34. Doi: 10.3892/Ijmm.2014.1722. Epub 2014 Apr 3. Icariin And Icaritin Stimulate The Proliferation Of Skbr3 Cells Through The Gper1-Mediated Modulation Of The Egf...
Hu Cp, Feng Jt, Tang Yl, Zhu Jq, Lin Mj, Yu Me. Mediators Inflamm. 2006;2006(5):84829. Lif Upregulates Expression Of Nk-1R In Nhbe Cells.
Tang Y, Zhang X, Qi F, Chen M, Li Y, Liu L, He W, Li Z, Zu X. Exp Ther Med. 2015 May;9(5):1851-1856. Epub 2015 Feb 25. Afatinib Inhibits Proliferation And Invasion And Promotes Apoptosis Of The T24 Bladder Cancer Cell Line.