SKU P02677
Size 100ug/vial
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Clone Number GFH-7
Host Rabbit
Applications WB

Overview

Product Name Anti-Phospho-GluR1 (S845) Rabbit Monoclonal Antibody
SKU/Catalog Number P02677
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Size 100ug/vial
Description Anti-Phospho-GluR1 (S845) Rabbit Monoclonal Antibody tested for WB in Human, Mouse, Rat
Cite This Product Anti-Phospho-GluR1 (S845) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P02677)
Host Rabbit
Immunogen A synthesized peptide derived from human GluR1
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

WB 1:500-1:1000

Images And Assay Conditions

/P/0/P02677 GRIA1 primary antibodies WB testing 1.jpg

Western blot analysis of Phospho-GluR1 (S845) expression in (1) Human brain lysate treated with Lambda phosphatase lysate; (2) Human brain lysate (P02677).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIA1 monoclonal antibody (Catalog # P02677) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRIA1

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P42261
Gene Name GRIA1
Protein Name Glutamate receptor 1
Tissue Specificity Detected in cerebellum (at protein level). .
Alternative Names Glutamate receptor 1;GluR-1;AMPA-selective glutamate receptor 1;GluR-A;GluR-K1;Glutamate receptor ionotropic, AMPA 1;GluA1;Gria1;Glur1;
Subcellular Localization Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density . Cell projection, dendrite . Cell projection, dendritic spine . Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression.
Molecular Weight 101579 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. .

Order Product (P02677)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Option Price
100ug $299
100ug+Free HRP Secondary BA1054 $299
100ug+Free Biotin Secondary BA1003 $299
30ug sample size $99

USD $299

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