Anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal Antibody
Rabbit IgG Monoclonal antibody for Phospho-JNK1/2/3 (T183+T183+T221) detection. Tested positive for WB, IHC, ICC/IF, IP, FC in Human, Mouse, Rat
|Reactivity||Human, Mouse, Rat|
|Applications||Flow Cytometry, IP, IF, IHC, ICC, WB|
|Product Name||Anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal Antibody
See all MAPK8 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.|
|Description||Rabbit IgG Monoclonal antibody for Phospho-JNK1/2/3 (T183+T183+T221) detection. Tested positive for WB, IHC, ICC/IF, IP, FC in Human, Mouse, Rat|
|Cite This Product||Anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MP02608)|
|Specificity||Anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal Antibody (MP02608) reacts with Human, Mouse, Rat MAPK8, in native form and recombinant. Superfamily members of MAPK8 are not reactive to MP02608.|
|Contents/Buffer||Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|Immunogen||A synthesized peptide derived from human Phospho-JNK1/2/3 (T183+T183+T221) Phospho-JNK1/2/3 (T183+T183+T221)|
|Reactivity||Human, Mouse, Rat|
Our Boster Quality Guarantee for Anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal Antibody covers its use in the following applications.
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Assay Dilutions Overview
Images And Assay Conditions
IF analysis of immunocytochemical section of NIH/3T3 cells treated with Anisomycin using anti- Phospho-JNK1/2/3 (T183+T183+T221) antibody (MP02608)
Phospho-JNK1/2/3 (T183+T183+T221) was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2Î¼g/mL rabbit anti- Phospho-JNK1/2/3 (T183+T183+T221) Antibody (MP02608) overnight at 4 °
IHC analysis of MAPK8 using anti-MAPK8 antibody (MP02608).
MAPK8 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAPK8 Antibody (MP02608) overnight at 4Â°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37Â°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Western blot analysis of MAPK8 using anti-MAPK8 antibody (MP02608).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK8 antigen affinity purified polyclonal antibody (Catalog # MP02608) at 0.5 ug/mL overnight at 4Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for MAPK8.
Protein Target Info (Source: Uniprot.org)
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Research Areas||Human, Mouse, Rat
*You can search these to find other products in these research areas.
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