Anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal Antibody

Rabbit IgG Monoclonal antibody for Phospho-JNK1/2/3 (T183+T183+T221) detection. Tested positive for WB, IHC, ICC/IF, IP, FC in Human, Mouse, Rat

SKU MP02608
Size 100ug
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Clone Number EAG-13
Host Rabbit
Applications Flow Cytometry, IP, IF, IHC, ICC, WB


Product Name Anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal Antibody
See all MAPK8 primary antibodies, ELISA kits and proteins
SKU/Catalog Number MP02608
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Size 100ug
Description Rabbit IgG Monoclonal antibody for Phospho-JNK1/2/3 (T183+T183+T221) detection. Tested positive for WB, IHC, ICC/IF, IP, FC in Human, Mouse, Rat
Cite This Product Anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MP02608)
Specificity Anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal Antibody (MP02608) reacts with Human, Mouse, Rat MAPK8, in native form and recombinant. Superfamily members of MAPK8 are not reactive to MP02608.
Host Rabbit
Contents/Buffer Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Immunogen A synthesized peptide derived from human Phospho-JNK1/2/3 (T183+T183+T221) Phospho-JNK1/2/3 (T183+T183+T221)
Reactivity Human, Mouse, Rat

Assay Details

Our Boster Quality Guarantee for Anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal Antibody covers its use in the following applications.

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
WB 1:500~1:2000
IHC 1:50~1:200
ICC/IF 1:50~1:200
IP 1:50

Images And Assay Conditions


IF analysis of immunocytochemical section of NIH/3T3 cells treated with Anisomycin using anti- Phospho-JNK1/2/3 (T183+T183+T221) antibody (MP02608)
Phospho-JNK1/2/3 (T183+T183+T221) was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti- Phospho-JNK1/2/3 (T183+T183+T221) Antibody (MP02608) overnight at 4 °


IHC analysis of MAPK8 using anti-MAPK8 antibody (MP02608).
MAPK8 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAPK8 Antibody (MP02608) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.


Western blot analysis of MAPK8 using anti-MAPK8 antibody (MP02608).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK8 antigen affinity purified polyclonal antibody (Catalog # MP02608) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for MAPK8.

Target Info

Protein Target Info (Source:

Uniprot Id P45983
Gene Name MAPK8

*if product is indicated to react with multiple species, protein info is based on the human gene.


Research Areas Human, Mouse, Rat

*You can search these to find other products in these research areas.

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)



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