SKU MP02608
Size 100ug
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Clone Number EAG-13
Host Rabbit
Applications Flow Cytometry, IP, IF, IHC, ICC, WB

Overview

Product Name Anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal Antibody
SKU/Catalog Number MP02608
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Size 100ug
Description Rabbit IgG Monoclonal antibody for Phospho-JNK1/2/3 (T183+T183+T221) detection. Tested positive for WB, IHC, ICC/IF, IP, FC in Human, Mouse, Rat
Cite This Product Anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MP02608)
Host Rabbit
Contents/Buffer Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Immunogen A synthesized peptide derived from human Phospho-JNK1/2/3 (T183+T183+T221) Phospho-JNK1/2/3 (T183+T183+T221)
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
WB 1:500~1:2000
IHC 1:50~1:200
ICC/IF 1:50~1:200
IP 1:50

Images And Assay Conditions

/M/P/MP02608 MAPK8 WB test 1.jpg

Western blot analysis of MAPK8 using anti-MAPK8 antibody (MP02608).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK8 antigen affinity purified polyclonal antibody (Catalog # MP02608) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for MAPK8.

/M/P/MP02608 MAPK8 IHC test 2.jpg

IHC analysis of MAPK8 using anti-MAPK8 antibody (MP02608).
MAPK8 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAPK8 Antibody (MP02608) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

/M/P/MP02608 MAPK8 IF test 3.jpg

IF analysis of immunocytochemical section of NIH/3T3 cells treated with Anisomycin using anti- Phospho-JNK1/2/3 (T183+T183+T221) antibody (MP02608)
Phospho-JNK1/2/3 (T183+T183+T221) was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti- Phospho-JNK1/2/3 (T183+T183+T221) Antibody (MP02608) overnight at 4 °C. DyLight®488 Conjugated Goat AntiRabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 °C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P45983
Gene Name MAPK8

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Research Areas Cancer, Cancer Susceptibility, Epigenetics And Nuclear Signaling, Immunology, Innate Immunity, Mapk Pathway, Oncoproteins, Oncoproteins/Suppressors, Protein Phosphorylation, Proto-Oncogenes, Ser / Thr Kinases, Serine/Threonine Kinases, Signal Transduction, Tlr Signaling, Transcription

*You can search these to find other products in these research areas.

Other Recommended Resources

Here are featured tools and databases that you might find useful.

Order Product (MP02608)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
100ug $299
100ug+Free HRP Secondary BA1054 $299
100ug+Free Biotin Secondary BA1003 $299
30ug sample size $99

USD $299

Ships in 7-10 business days.

Troubleshooting

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

We promise all of our products perform as described in datasheets.

Write a review for MP02608

Publications

Therapeutic effects of 1,25-dihydroxyvitamin D3 on diabetes-induced liver complications in a rat model
Recombinant Newcastle disease virus (rL-RVG) triggers autophagy and apoptosis in gastric carcinoma cells by inducing ER stress
Cui Y, Wu W, Lv P, Zhang J, Bai B, Cao W. Oncotarget. 2017 Dec 11;9(1):783-790. doi: 10.18632/oncotarget.23153. eCollection 2018 Jan 2. Down-regulation of long non-coding RNA ESCCAL_1 inhibits tumor growth of esophageal squamous cell carcinoma in ...
Ding Y, Zou J, Li Z, Tian J, Abdelalim S, Du F, She R, Wang D, Tan C, Wang H, Chen W, Lv D, Chang L. Plos One. 2011;6(5):E20008. Doi: 10.1371/Journal.Pone.0020008. Epub 2011 May 23. Study Of Histopathological And Molecular Changes Of Rat Kidney Un...