Anti-Phospho-Net (Ser357) Polyclonal Antibody

ELK3 antibody

Boster Bio Anti-Phospho-Net (Ser357) Polyclonal Antibody (Catalog # P06026). Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Monkey.

Product Info Summary

SKU: P06026
Size: 100 µl
Reactive Species: Human, Monkey, Mouse
Host: Rabbit
Application: ELISA, IF, IHC, WB

Product Name

Anti-Phospho-Net (Ser357) Polyclonal Antibody

View all ELK3 Antibodies

SKU/Catalog Number

P06026

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-Net (Ser357) Polyclonal Antibody (Catalog # P06026). Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Monkey.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-Net (Ser357) Polyclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P06026)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Immunogen

Synthesized peptide derived from human Net around the phosphorylation site of S357.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P06026 is reactive to ELK3 in Human, Monkey, Mouse

Applications

P06026 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

44.24kDa

Background of ELK3

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.

Ferrer, et al. (2003) Neuropathology & Applied Neurobiology 29: 23
Zhonghong Guan, et al. (1999) J Biol Chem, Vol. 274: 36200-36206
D.Margriet Ouwens1, et al. (2002)The EMBO Journal 21: 3782

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1/500 - 1/2000
Immunohistochemistry: 1/100 - 1/300
Immunofluorescence: 1/200 - 1/1000
ELISA: 1/20000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For ELK3 (Source: Uniprot.org, NCBI)

Gene Name

ELK3

Full Name

ETS domain-containing protein Elk-3

Weight

44.24kDa

Superfamily

ETS family

Alternative Names

ELK3, ETS-domain protein (SRF accessory protein 2); ERPETS domain-containing protein Elk-3; ETS-domain protein; ETS-related protein ERP; NETSRF accessory protein 2; SAP-2; SAP2ETS-related protein NET; Serum response factor accessory protein 2 ELK3 ERP, NET, SAP-2, SAP2 ETS transcription factor ELK3 ETS domain-containing protein Elk-3|ELK3, ETS transcription factor|ELK3, ETS-domain protein (SRF accessory protein 2)|ETS-related protein ERP|ETS-related protein NET|SRF accessory protein 2|serum response factor accessory protein 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ELK3, check out the ELK3 Infographic

ELK3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ELK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-Phospho-Net (Ser357) Polyclonal Antibody

Question

Do you require any kind of licensing agreement with the researcher who wants to evaluate a Boster polyclonal antibody for possible inclusion in a commercialize product?

Verified customer

Asked: 2022-07-05

Answer

Yes, we need to have a licensing agreement, for many purposes but primarily to ensure the continuous supply of this product, which the researcher would need. We should ask them to discuss more details including the expected demand of this antibody. Since this is a polyclonal antibody we need to produce ahead of time and validate to ensure it keeps working properly for the customer.

Boster Scientific Support

Answered: 2022-07-05

Question

My question regarding product P06026, anti-Phospho-Net (Ser357) Polyclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-12-24

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P06026 anti-Phospho-Net (Ser357) Polyclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-12-24

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-Phospho-Net (Ser357) Polyclonal antibody P06026. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-23

Question

I see that the anti-Phospho-Net (Ser357) Polyclonal antibody P06026 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-07-10

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-07-10

Question

We are currently using anti-Phospho-Net (Ser357) Polyclonal antibody P06026 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, monkey, mouse. Is it likely that the antibody can work on canine tissues as well?

M. Jones

Verified customer

Asked: 2015-05-06

Answer

The anti-Phospho-Net (Ser357) Polyclonal antibody (P06026) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-05-06

Order DetailsPrice
P06026-100uL

100uL

$360

Free Secondary Antibody

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P06026
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

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