Anti-Phospho-STAT3 (S727) Rabbit Monoclonal Antibody

STAT3 antibody

Boster Bio Anti-Phospho-STAT3 (S727) Rabbit Monoclonal Antibody catalog # P00007-1. Tested in IP, IF, WB applications. This antibody reacts with Human, Rat. Cited in 5 publication(s).

Product Info Summary

SKU: P00007-1
Size: 100 μl
Reactive Species: Human, Rat
Host: Rabbit
Application: IP, IF, IHC, ICC, WB

Product Name

Anti-Phospho-STAT3 (S727) Rabbit Monoclonal Antibody

View all STAT3 Antibodies

SKU/Catalog Number

P00007-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Phospho-STAT3 (S727) Rabbit Monoclonal Antibody catalog # P00007-1. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-STAT3 (S727) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00007-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

CAA-19

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Phospho-STAT3 (S727)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P00007-1 is reactive to STAT3 in Human, Rat

Applications

P00007-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For STAT3 (Source: Uniprot.org, NCBI)

Uniprot ID

P40763

Gene ID

6774

Gene Name

STAT3

Full Name

Signal transducer and activator of transcription 3

Weight

88.068kDa

Superfamily

transcription factor STAT family

Alternative Names

Acute-phase response factor; APRFMGC16063; DNA-binding protein APRF; FLJ20882; HIES; signal transducer and activator of transcription 3 (acute-phase responsefactor); signal transducer and activator of transcription 3; STAT3 STAT3 ADMIO, ADMIO1, APRF, HIES signal transducer and activator of transcription 3 signal transducer and activator of transcription 3|DNA-binding protein APRF|acute-phase response factor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on STAT3, check out the STAT3 Infographic

STAT3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for STAT3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

P00007-1 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Zhang T,Ma L,Wu P,Li W,Li T,Gu R,Dan X,Li Z,Fan X,Xiao Z.Gallic acid has anticancer activity and enhances the anticancer effects of cisplatin in non‑small cell lung cancer A549 cells via the JAK/STAT3 signaling pathway.Oncol Rep.2019 Mar;41(3):1779-1788.doi:10.3892/or.2019.6976.Epub 2019 Jan 22.PMID:30747218.
Species: Human
P00007-1 usage in article: APP:WB, SAMPLE:A549 CELL, DILUTION:1:1000

Protective effects of Tongxinluo on cerebral ischemia/reperfusion injury related to Connexin 43/Calpain II/Bax/Caspase-3 pathway in rat

IL-6 Inhibits Starvation-induced Autophagy via the STAT3/Bcl-2 Signaling Pathway

Prognostic role of STAT3 in solid tumors: a systematic review and meta-analysis

Khan M, Li T, Ahmad Khan Mk, Rasul A, Nawaz F, Sun M, Zheng Y, Ma T. Biomed Res Int. 2013;2013:719858. Doi: 10.1155/2013/719858. Epub 2012 Dec 27. Alantolactone Induces Apoptosis In Hepg2 Cells Through Gsh Depletion, Inhibition Of Stat3 Activation...

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5 Customer Q&As for Anti-Phospho-STAT3 (S727) Rabbit Monoclonal Antibody

Question

We are currently using anti-Phospho-STAT3 (S727) Rabbit Monoclonal antibody P00007-1 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-24

Answer

The anti-Phospho-STAT3 (S727) Rabbit Monoclonal antibody (P00007-1) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-24

Question

My boss were well pleased with the WB result of your anti-Phospho-STAT3 (S727) Rabbit Monoclonal antibody. However we have been able to see positive staining in kidney pancreas cytoplasm. nucleus using this antibody. Is that expected? Could you tell me where is STAT3 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-12-09

Answer

From literature, kidney pancreas does express STAT3. Generally STAT3 expresses in cytoplasm. nucleus. Regarding which tissues have STAT3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Erythroleukemia, Pubmed ID: 23186163
Kidney, and Pancreas, Pubmed ID: 15489334
Liver, Pubmed ID: 7701321, 24275569
Placenta, Pubmed ID: 7512451

Boster Scientific Support

Answered: 2019-12-09

Question

We purchased anti-Phospho-STAT3 (S727) Rabbit Monoclonal antibody for IP on cervix carcinoma last year. I am using rat, and We intend to use the antibody for IF next. We want examining cervix carcinoma as well as upper lobe of lung in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?

D. Mitchell

Verified customer

Asked: 2018-12-11

Answer

I looked at the website and datasheets of our anti-Phospho-STAT3 (S727) Rabbit Monoclonal antibody and it seems that P00007-1 has been tested on rat in both IP and IF. Thus P00007-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-12-11

Question

you antibody using your anti-Phospho-STAT3 (S727) Rabbit Monoclonal antibody for response to peptide hormone studies. Has this antibody been tested with western blotting on a431 cell lysate? We would like to see some validation images before ordering.

C. Jones

Verified customer

Asked: 2016-11-28

Answer

I appreciate your inquiry. This P00007-1 anti-Phospho-STAT3 (S727) Rabbit Monoclonal antibody is validated on a431 cell lysate. It is guaranteed to work for IP, IF, WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2016-11-28

Question

We have been able to see staining in human upper lobe of lung. Are there any suggestions? Is anti-Phospho-STAT3 (S727) Rabbit Monoclonal antibody supposed to stain upper lobe of lung positively?

D. Moore

Verified customer

Asked: 2013-11-11

Answer

From literature upper lobe of lung does express STAT3. From Uniprot.org, STAT3 is expressed in upper lobe of lung, placenta, kidney pancreas, liver, cervix carcinoma, erythroleukemia, among other tissues. Regarding which tissues have STAT3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Erythroleukemia, Pubmed ID: 23186163
Kidney, and Pancreas, Pubmed ID: 15489334
Liver, Pubmed ID: 7701321, 24275569
Placenta, Pubmed ID: 7512451

Boster Scientific Support

Answered: 2013-11-11

Size

Total: $315

SKU:P00007-1

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P00007-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

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