SKU P03794
Size 100ug/vial
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Clone Number FCE-19
Host Rabbit
Applications IHC, WB

Overview

Product Name Anti-Phospho-Synapsin I (S9) Rabbit Monoclonal Antibody
SKU/Catalog Number P03794
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Size 100ug/vial
Description Anti-Phospho-Synapsin I (S9) Rabbit Monoclonal Antibody tested for IHC, WB in Human, Mouse, Rat
Cite This Product Anti-Phospho-Synapsin I (S9) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03794)
Host Rabbit
Immunogen A synthesized peptide derived from human Phospho-Synapsin I (S9)
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

WB 1:500-1:2000
IHC 1:50-1:200

Images And Assay Conditions

/P/0/P03794 SYN1 primary antibodies WB testing 1.jpg

Western blot analysis of Phospho-Synapsin I (S9) expression in (1) Human brain lysate; (2) Human brain lysate treated with AP (P03794).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYN1 monoclonal antibody (Catalog # P03794) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SYN1

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P17600
Gene Name SYN1
Protein Name Synapsin-1
Alternative Names Synapsin-1;Synapsin I;Syn1;Syn-1;
Subcellular Localization Cell junction, synapse . Golgi apparatus .
Molecular Weight 74097 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. Regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxide functions at a presynaptic level.

Order Product (P03794)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Option Price
100ug $299
100ug+Free HRP Secondary BA1054 $299
100ug+Free Biotin Secondary BA1003 $299
30ug sample size $99

USD $299

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Publications

Neurotoxicity induced by zinc oxide nanoparticles: age-related differences and interaction
S100+cells: A new neuro-immune cross-talkers in lymph organs