Product Info Summary
SKU: | P00235 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IP, WB |
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Product info
Product Name
Anti-Phospho-Vimentin (S72) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00235
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Vimentin (S72) Rabbit Monoclonal Antibody catalog # P00235. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Vimentin (S72) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00235)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADE-22
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-Vimentin (S72)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00235 is reactive to VIM in Human, Mouse, Rat
Applications
P00235 is guaranteed for IP, WB Boster Guarantee
Observed Molecular Weight
100-110 kDa
Calculated molecular weight
53.652kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:50
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Phospho-Vimentin (Ser72) in HeLa cell lysates treated with Calyculin A.
Protein Target Info & Infographic
Gene/Protein Information For VIM (Source: Uniprot.org, NCBI)
Gene Name
VIM
Full Name
Vimentin
Weight
53.652kDa
Superfamily
intermediate filament family
Alternative Names
FLJ36605; VIM; Vimentin VIM vimentin vimentin|epididymis secretory sperm binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VIM, check out the VIM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VIM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Vimentin (S72) Rabbit Monoclonal Antibody (P00235)
Hello CJ!
P00235 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The Role of the RhoA/ROCK Signaling Pathway in Mechanical Strain-Induced Scleral Myofibroblast Differentiation
Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments
Autophagy Is Indispensable for the Self-Renewal and Quiescence of Ovarian Cancer Spheroid Cells with Stem Cell-Like Properties
Expression of E-cadherin and vimentin in oral squamous cell carcinoma
%u03B22-microglobulin induces epithelial-mesenchymal transition in human renal proximal tubule epithelial cells in vitro
Distinct Roles For ROCK1 and ROCK2 in the Regulation of Oxldl-Mediated Endothelial Dysfunction
MTDH promotes glioma invasion through regulating miR-130b-ceRNAs
Micro-/nano-topography of selective laser melting titanium enhances adhesion and proliferation and regulates adhesion-related gene expressions of human %u2026
Mesenchymal Stem Cells Promote Tumor Progression via Inducing Stroma Remodeling on Rabbit VX2 Bladder Tumor Model
Changes in hippocampal ultrastructure and vimentin expression in rhesus monkeys following selective deep hypothermia and blood occlusion
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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4 Customer Q&As for Anti-Phospho-Vimentin (S72) Rabbit Monoclonal Antibody
Question
We have seen staining in human cajal-retzius cell fetal brain cortex. What should we do? Is anti-Phospho-Vimentin (S72) Rabbit Monoclonal antibody supposed to stain cajal-retzius cell fetal brain cortex positively?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
From what I have seen in literature cajal-retzius cell fetal brain cortex does express VIM. From what I have seen in Uniprot.org, VIM is expressed in dorsal root ganglion, lymphoma, testis, embryo, placenta stomach, adipose tissue coronary artery, cervix, placenta testis, mammary carcinoma, t-cell, hepatoma, brain, cajal-retzius cell fetal brain cortex, fibroblast, osteosarcoma, lymphoblast, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have VIM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryo, Placenta, and Stomach, Pubmed ID: 14702039
Fibroblast, Pubmed ID: 3371665
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Lymphoma, Pubmed ID: 14996095
Mammary carcinoma, Pubmed ID: 9150946
Osteosarcoma, Pubmed ID: 2323579
Boster Scientific Support
Answered: 2020-01-27
Question
We were well pleased with the WB result of your anti-Phospho-Vimentin (S72) Rabbit Monoclonal antibody. However we have observed positive staining in osteosarcoma cytoplasm using this antibody. Is that expected? Could you tell me where is VIM supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-02-14
Answer
According to literature, osteosarcoma does express VIM. Generally VIM expresses in cytoplasm. Regarding which tissues have VIM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryo, Placenta, and Stomach, Pubmed ID: 14702039
Fibroblast, Pubmed ID: 3371665
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Lymphoma, Pubmed ID: 14996095
Mammary carcinoma, Pubmed ID: 9150946
Osteosarcoma, Pubmed ID: 2323579
Boster Scientific Support
Answered: 2018-02-14
Question
We are currently using anti-Phospho-Vimentin (S72) Rabbit Monoclonal antibody P00235 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-01
Answer
The anti-Phospho-Vimentin (S72) Rabbit Monoclonal antibody (P00235) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-01
Question
We ordered your anti-Phospho-Vimentin (S72) Rabbit Monoclonal antibody for WB on fibroblast in the past. I am using human, and We want to use the antibody for IP next. We want examining fibroblast as well as adipose tissue coronary artery in our next experiment. Do you have any suggestion on which antibody would work the best for IP?
W. Kulkarni
Verified customer
Asked: 2014-07-18
Answer
I viewed the website and datasheets of our anti-Phospho-Vimentin (S72) Rabbit Monoclonal antibody and I see that P00235 has been tested on human in both WB and IP. Thus P00235 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-07-18