Anti-Phospho-Vimentin (S72) Rabbit Monoclonal Antibody

Vimentin antibody

Boster Bio Anti-Phospho-Vimentin (S72) Rabbit Monoclonal Antibody catalog # P00235. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: P00235
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IP, WB

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Product Name

Anti-Phospho-Vimentin (S72) Rabbit Monoclonal Antibody

View all Vimentin Antibodies

SKU/Catalog Number

P00235

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Phospho-Vimentin (S72) Rabbit Monoclonal Antibody catalog # P00235. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-Vimentin (S72) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00235)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ADE-22

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Phospho-Vimentin (S72)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P00235 is reactive to VIM in Human, Mouse, Rat

Applications

P00235 is guaranteed for IP, WB Boster Guarantee

Observed Molecular Weight

100-110 kDa

Calculated molecular weight

53.652kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For VIM (Source: Uniprot.org, NCBI)

Gene Name

VIM

Full Name

Vimentin

Weight

53.652kDa

Superfamily

intermediate filament family

Alternative Names

FLJ36605; VIM; Vimentin VIM vimentin vimentin|epididymis secretory sperm binding protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on VIM, check out the VIM Infographic

VIM infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for VIM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

P00235 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The Role of the RhoA/ROCK Signaling Pathway in Mechanical Strain-Induced Scleral Myofibroblast Differentiation

Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments

Autophagy Is Indispensable for the Self-Renewal and Quiescence of Ovarian Cancer Spheroid Cells with Stem Cell-Like Properties

Expression of E-cadherin and vimentin in oral squamous cell carcinoma

%u03B22-microglobulin induces epithelial-mesenchymal transition in human renal proximal tubule epithelial cells in vitro

Distinct Roles For ROCK1 and ROCK2 in the Regulation of Oxldl-Mediated Endothelial Dysfunction

MTDH promotes glioma invasion through regulating miR-130b-ceRNAs

Micro-/nano-topography of selective laser melting titanium enhances adhesion and proliferation and regulates adhesion-related gene expressions of human %u2026

Mesenchymal Stem Cells Promote Tumor Progression via Inducing Stroma Remodeling on Rabbit VX2 Bladder Tumor Model

Changes in hippocampal ultrastructure and vimentin expression in rhesus monkeys following selective deep hypothermia and blood occlusion

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4 Customer Q&As for Anti-Phospho-Vimentin (S72) Rabbit Monoclonal Antibody

Question

We have seen staining in human cajal-retzius cell fetal brain cortex. What should we do? Is anti-Phospho-Vimentin (S72) Rabbit Monoclonal antibody supposed to stain cajal-retzius cell fetal brain cortex positively?

Verified Customer

Verified customer

Asked: 2020-01-27

Answer

From what I have seen in literature cajal-retzius cell fetal brain cortex does express VIM. From what I have seen in Uniprot.org, VIM is expressed in dorsal root ganglion, lymphoma, testis, embryo, placenta stomach, adipose tissue coronary artery, cervix, placenta testis, mammary carcinoma, t-cell, hepatoma, brain, cajal-retzius cell fetal brain cortex, fibroblast, osteosarcoma, lymphoblast, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have VIM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryo, Placenta, and Stomach, Pubmed ID: 14702039
Fibroblast, Pubmed ID: 3371665
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Lymphoma, Pubmed ID: 14996095
Mammary carcinoma, Pubmed ID: 9150946
Osteosarcoma, Pubmed ID: 2323579

Boster Scientific Support

Answered: 2020-01-27

Question

We were well pleased with the WB result of your anti-Phospho-Vimentin (S72) Rabbit Monoclonal antibody. However we have observed positive staining in osteosarcoma cytoplasm using this antibody. Is that expected? Could you tell me where is VIM supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-02-14

Answer

According to literature, osteosarcoma does express VIM. Generally VIM expresses in cytoplasm. Regarding which tissues have VIM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryo, Placenta, and Stomach, Pubmed ID: 14702039
Fibroblast, Pubmed ID: 3371665
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Lymphoma, Pubmed ID: 14996095
Mammary carcinoma, Pubmed ID: 9150946
Osteosarcoma, Pubmed ID: 2323579

Boster Scientific Support

Answered: 2018-02-14

Question

We are currently using anti-Phospho-Vimentin (S72) Rabbit Monoclonal antibody P00235 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2018-02-01

Answer

The anti-Phospho-Vimentin (S72) Rabbit Monoclonal antibody (P00235) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-02-01

Question

We ordered your anti-Phospho-Vimentin (S72) Rabbit Monoclonal antibody for WB on fibroblast in the past. I am using human, and We want to use the antibody for IP next. We want examining fibroblast as well as adipose tissue coronary artery in our next experiment. Do you have any suggestion on which antibody would work the best for IP?

W. Kulkarni

Verified customer

Asked: 2014-07-18

Answer

I viewed the website and datasheets of our anti-Phospho-Vimentin (S72) Rabbit Monoclonal antibody and I see that P00235 has been tested on human in both WB and IP. Thus P00235 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2014-07-18

Order DetailsPrice
P00235

100μL

$370
P00235-30ul

30μL sample

$99
P00235-carrier-free

Carrier Free

$370

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