|Validated Species:||All Animals, Human|
Data & Images
|Product Name||Anti-Phosphoserine Antibody (Monoclonal, PSR-45)|
|Description||Mouse IgG monoclonal antibody for Phosphoserine detection. Tested with WB in Human; all kinds of animals.No cross reactivity with otherproteins.|
|Cite This Product||Anti-Phosphoserine Antibody (Monoclonal, PSR-45) PSR-45 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1084)|
|Replacement Item||This antibody may replace the following items: sc-74829|sc-74827|sc-365183|sc-271421|sc-98683|sc-69704 from Santa Cruz Biotechnology.|
|Validated Species||All Animals, Human|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Phosphoserine conjugated to Keyhole Limpet Hemocyanin(KLH).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Phosphoserine phosphatase|
|Molecular Weight||24968 MW|
|Protein Function||Catalyzes the last step in the biosynthesis of serine from carbohydrates. The reaction mechanism proceeds via the formation of a phosphoryl-enzyme intermediates (By similarity). .|
|Tissue Specificity||PSAT1: Expressed at high levels in the brain, liver,kidney and pancreas, and very weakly expressed in the thymus,prostate, testis and colon.|
|Alternative Names||Phosphoserine phosphatase;PSP;PSPase;22.214.171.124;O-phosphoserine phosphohydrolase;Psph;|
|Research Areas|||signal transduction|metabolism|amino acids| metabolism|pathways and processes|metabolic signaling pathways|amino acid metabolism||
Background for Phosphoserine phosphatase
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Phosphoserine Antibody (Monoclonal, PSR-45) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 1-2μg/ml, Human, all kinds of animals|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Phosphoserine Antibody (Monoclonal, PSR-45) Images
Click the images to enlarge.
All lanes: Anti Phosphoserine (MA1084) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 45KD
Observed bind size: 45KD