SKU MA1084
Size 100μg/vial
Reactivity All Animals, Human
Clonality Monoclonal
Clone Number PSR-45
Host Mouse
Ig Isotype Mouse IgG1
Applications WB

Overview

Product Name Anti-Phosphoserine Antibody (Monoclonal, PSR-45)
SKU/Catalog Number MA1084
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Phosphoserine detection. Tested with WB in Human; all kinds of animals.No cross reactivity with otherproteins.
Cite This Product Anti-Phosphoserine Antibody (Monoclonal, PSR-45) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1084)
Host Mouse
Immunogen Phosphoserine conjugated to Keyhole Limpet Hemocyanin(KLH).
Reactivity All Animals, Human

Assay Details

Assay Dilutions Overview

Western blot, 1-2μg/ml, Human, all kinds of animals

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.

Images And Assay Conditions

/antibody/ma1084 1_1 WB anti phosphoserine antibody monoclonal psr 45.jpg

Anti-Phosphoserine antibody (monoclonal), MA1084, Western blotting
All lanes: Anti Phosphoserine (MA1084) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 45KD
Observed bind size: 45KD

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q5M819
Gene Name Psph
Protein Name Phosphoserine phosphatase
Tissue Specificity PSAT1: Expressed at high levels in the brain, liver,kidney and pancreas, and very weakly expressed in the thymus,prostate, testis and colon.
Alternative Names Phosphoserine phosphatase;PSP;PSPase;3.1.3.3;O-phosphoserine phosphohydrolase;Psph;
Molecular Weight 24968 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Catalyzes the last step in the biosynthesis of serine from carbohydrates. The reaction mechanism proceeds via the formation of a phosphoryl-enzyme intermediates (By similarity). .
Background Phosphoserine phosphatase fills an important role in the biosynthesis of serine from carbohydrates by catalyzing the last step, hydrolysis of O-phosphoserine. Phosphoserine phosphatase activity in lymphoblasts and fibroblasts was reduced to 25% of normal values. The human phosphoserine gene(PSP)is mapped to the pter-q22 region of chromosome 7.

Order Product (MA1084)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $240
100ug+Free HRP Secondary BA1050 $240
100ug+Free Biotin Secondary BA1001 $240
Ships in 5-7 business days.

Troubleshooting

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Publications

Li LU, Zhao Y, Zhang H. Cell Death Dis. 2017 Apr 20;8(4):e2752. doi: 10.1038/cddis.2017.169. P16INK4a upregulation mediated by TBK1 induces retinal ganglion cell senescence in ischemic injury.

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.