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SKU:MA1084
Clonality:Monoclonal
Clone Number:PSR-45
Application:WB
Price: $200.00
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Overview

Product Name Anti-Phosphoserine Antibody (Monoclonal, PSR-45)
Description Mouse IgG monoclonal antibody for Phosphoserine detection. Tested with WB in Human; all kinds of animals.No cross reactivity with otherproteins.
Cite This Product Anti-Phosphoserine Antibody (Monoclonal, PSR-45) PSR-45 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1084)
Replacement Item This antibody may replace the following items: sc-74829|sc-74827|sc-365183|sc-271421|sc-98683|sc-69704 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species All Animals, Human
Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Phosphoserine conjugated to Keyhole Limpet Hemocyanin(KLH).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number PSR-45
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Psph
Protein Name Phosphoserine phosphatase
Molecular Weight 24968 MW
Protein Function Catalyzes the last step in the biosynthesis of serine from carbohydrates. The reaction mechanism proceeds via the formation of a phosphoryl-enzyme intermediates (By similarity). .
Tissue Specificity PSAT1: Expressed at high levels in the brain, liver,kidney and pancreas, and very weakly expressed in the thymus,prostate, testis and colon.
Uniprot ID Q5M819
Alternative Names Phosphoserine phosphatase;PSP;PSPase;3.1.3.3;O-phosphoserine phosphohydrolase;Psph;
Research Areas |signal transduction|metabolism|amino acids| metabolism|pathways and processes|metabolic signaling pathways|amino acid metabolism|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Phosphoserine phosphatase

Phosphoserine phosphatase fills an important role in the biosynthesis of serine from carbohydrates by catalyzing the last step, hydrolysis of O-phosphoserine. Phosphoserine phosphatase activity in lymphoblasts and fibroblasts was reduced to 25% of normal values. The human phosphoserine gene(PSP)is mapped to the pter-q22 region of chromosome 7.

Anti-Phosphoserine Antibody (Monoclonal, PSR-45) Images

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Anti-Phosphoserine Antibody (Monoclonal, PSR-45)
Anti-Phosphoserine antibody (monoclonal), MA1084, Western blotting
All lanes: Anti Phosphoserine (MA1084) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 45KD
Observed bind size: 45KD
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Publications

Li LU, Zhao Y, Zhang H. Cell Death Dis. 2017 Apr 20;8(4):e2752. doi: 10.1038/cddis.2017.169. P16INK4a upregulation mediated by TBK1 induces retinal ganglion cell senescence in ischemic injury.

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.