Anti-PI 3 Kinase p85 alpha/PIK3R1 Antibody

PI 3-Kinase p85 alpha antibody

Boster Bio Anti-PI 3 Kinase p85 alpha/PIK3R1 Antibody catalog # PA1410. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: PA1410
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-PI 3 Kinase p85 alpha/PIK3R1 Antibody

View all PI 3-Kinase p85 alpha Antibodies

SKU/Catalog Number

PA1410

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PI 3 Kinase p85 alpha/PIK3R1 Antibody catalog # PA1410. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PI 3 Kinase p85 alpha/PIK3R1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1410)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human PI 3 Kinase p85 alpha, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1410 is reactive to PIK3R1 in Human, Mouse, Rat

Applications

PA1410 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

85 kDa

Calculated molecular weight

83.598kDa

Background of PI 3-Kinase p85 alpha

Phosphatidylinositol 3-kinase regulatory subunit alpha is an enzyme that in humans is encoded by the PIK3R1 gene. Its gene is mapped to 5q13. the bovine PI3K p85 subunit consists of 2 closely related proteins, p85-alpha and p85-beta. They cloned cDNAs encoding both p85 subunits, each of which is a 724-amino acid polypeptide. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For PIK3R1 (Source: Uniprot.org, NCBI)

Gene Name

PIK3R1

Full Name

Phosphatidylinositol 3-kinase regulatory subunit alpha

Weight

83.598kDa

Superfamily

PI3K p85 subunit family

Alternative Names

GRB1; GRB1PI3-kinase subunit p85-alpha; p85; p85-ALPHA; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; phosphatidylinositol 3-kinase regulatory subunit alpha; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphatidylinositol 3-kinase, regulatory, 1; phosphatidylinositol 3-kinase-associated p-85 alpha; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); PI 3Kinase p85 alpha; PI 3-Kinase p85 alpha; PI3K regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PIK3R1; PtdIns-3-kinase regulatory subunit alpha; PtdIns-3-kinase regulatory s PIK3R1 AGM7, GRB1, IMD36, p85, p85-ALPHA phosphoinositide-3-kinase regulatory subunit 1 phosphatidylinositol 3-kinase regulatory subunit alpha|PI3-kinase subunit p85-alpha|PI3K regulatory subunit alpha|phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha|phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)|phosphatidylinositol 3-kinase-associated p-85 alpha|phosphoinositide-3-kinase regulatory subunit alpha|phosphoinositide-3-kinase, regulatory subunit 1 (alpha)|ptdIns-3-kinase regulatory subunit alpha

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PIK3R1, check out the PIK3R1 Infographic

PIK3R1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PIK3R1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1410 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Ren,K.,Chi,X.,Wu,T.et al.Preliminary Study on the Antibacterial and Cytotoxic Effects of the Synthetic New Peptide NJP9-A.Int J Pept Res Ther(2021).https://doi.org/10.1007/s10989-021-10246-4
Species: Human
PA1410 usage in article: APP:WB, SAMPLE:K562 CELL AND NALM-6 CELL, DILUTION:NA

Expressions Profiles of the Proteins Associated with Carbohydrate Metabolism in Rat Liver Regeneration

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5 Customer Q&As for Anti-PI 3 Kinase p85 alpha/PIK3R1 Antibody

Question

We are currently using anti-PI 3 Kinase p85 alpha/PIK3R1 antibody PA1410 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-13

Answer

The anti-PI 3 Kinase p85 alpha/PIK3R1 antibody (PA1410) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-13

Question

Does PA1410 anti-PI 3 Kinase p85 alpha/PIK3R1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-04-08

Answer

It shows on the product datasheet, PA1410 anti-PI 3 Kinase p85 alpha/PIK3R1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-04-08

Question

What COLO cell lysate was used for WB testing of PA1410?

Verified customer

Asked: 2019-03-30

Answer

COLO-320 whole cell lysate was used in WB for the Anti-PI 3 Kinase P85 Alpha/PIK3R1 Antibody (PA1410).

Boster Scientific Support

Answered: 2019-04-02

Question

I see that the anti-PI 3 Kinase p85 alpha/PIK3R1 antibody PA1410 works with WB, what is the protocol used to produce the result images on the product page?

D. Brown

Verified customer

Asked: 2019-01-08

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-01-08

Question

Will anti-PI 3 Kinase p85 alpha/PIK3R1 antibody PA1410 work on primate WB with skeletal muscle?

Verified Customer

Verified customer

Asked: 2017-12-14

Answer

Our lab technicians have not tested anti-PI 3 Kinase p85 alpha/PIK3R1 antibody PA1410 on primate. You can run a BLAST between primate and the immunogen sequence of anti-PI 3 Kinase p85 alpha/PIK3R1 antibody PA1410 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate skeletal muscle in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-12-14

Order DetailsPrice
PA1410

100μg

$370
PA1410-10ug

10μg sample (liquid)

$99
PA1410-Biotin

100 μg Biotin conjugated

$570
PA1410-Cy3

100 μg Cy3 conjugated

$570
PA1410-Dylight488

100 μg Dylight488 conjugated

$570
PA1410-Dylight550

100 μg Dylight550 conjugated

$570
PA1410-Dylight594

100 μg Dylight594 conjugated

$570
PA1410-FITC

100 μg FITC conjugated

$570
PA1410-HRP

100 μg HRP conjugated

$570
PA1410-APC

100 μg APC conjugated

$670
PA1410-PE

100 μg PE conjugated

$670
PA1410-carrier-free

Carrier Free

$370

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