Anti-E3 SUMO-protein ligase PIAS1 PIAS1 Antibody

PIAS1 antibody

Boster Bio Anti-E3 SUMO-protein ligase PIAS1 PIAS1 Antibody catalog # PA2252. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: PA2252
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, ICC, WB

Product Name

Anti-E3 SUMO-protein ligase PIAS1 PIAS1 Antibody

View all PIAS1 Antibodies

SKU/Catalog Number

PA2252

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-E3 SUMO-protein ligase PIAS1 PIAS1 Antibody catalog # PA2252. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-E3 SUMO-protein ligase PIAS1 PIAS1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2252)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human PIAS1, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2252 is reactive to PIAS1 in Human, Mouse, Rat

Applications

PA2252 is guaranteed for IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

100 kDa

Calculated molecular weight

71.836kDa

Background of PIAS1

E3 SUMO-protein ligase PIAS1 is an enzyme that in humans is encoded by the PIAS1 gene. It is mapped to 15q23. This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. PIAS1 inhibited STAT1-mediated gene activation in response to interferon when expressed in mammalian cells. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. On the other hand, PIAS1 also can function as a SUMO ligase, or possibly as a tightly bound regulator of it, toward p53.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For PIAS1 (Source: Uniprot.org, NCBI)

Gene Name

PIAS1

Full Name

E3 SUMO-protein ligase PIAS1

Weight

71.836kDa

Superfamily

PIAS family

Alternative Names

AR interacting protein; DDXBP1MGC141878; DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1; DEAD/H box-binding protein 1; E3 SUMO-protein ligase PIAS1; GBPRNA helicase II-binding protein; GU/RH-II; Gu-binding protein; MGC141879; Protein inhibitor of activated STAT protein 1; protein inhibitor of activated STAT, 1; protein inhibitor of activated STAT-1; zinc finger, MIZ-type containing 3; ZMIZ3 PIAS1 DDXBP1, GBP, GU/RH-II, ZMIZ3 protein inhibitor of activated STAT 1 E3 SUMO-protein ligase PIAS1|AR interacting protein|DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1|DEAD/H box-binding protein 1|E3 SUMO-protein transferase PIAS1|RNA helicase II-binding protein|gu-binding protein|protein inhibitor of activated STAT protein 1|zinc finger, MIZ-type containing 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PIAS1, check out the PIAS1 Infographic

PIAS1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PIAS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA2252 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Scortegagna M1, Berthon A2, Settas N2, Giannakou A2, Garcia G1, Li JL1, James B1, Liddington RC1, Vilches-Moure JG3, Stratakis CA2, Ronai ZA1,4. JCI Insight. 2017 Dec 7;2(23). pii: 97128. doi: 10.1172/jci.insight.97128. [Epub ahead of print] The E...

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1 Customer Q&As for Anti-E3 SUMO-protein ligase PIAS1 PIAS1 Antibody

Question

We are currently using anti-PIAS1 antibody PA2252 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-12

Answer

The anti-PIAS1 antibody (PA2252) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-12

Order DetailsPrice
PA2252

100μg

$370
PA2252-10ug

10μg sample (liquid)

$99
PA2252-Biotin

100 μg Biotin conjugated

$570
PA2252-Cy3

100 μg Cy3 conjugated

$570
PA2252-Dylight488

100 μg Dylight488 conjugated

$570
PA2252-Dylight550

100 μg Dylight550 conjugated

$570
PA2252-Dylight594

100 μg Dylight594 conjugated

$570
PA2252-FITC

100 μg FITC conjugated

$570
PA2252-HRP

100 μg HRP conjugated

$570
PA2252-APC

100 μg APC conjugated

$670
PA2252-PE

100 μg PE conjugated

$670
PA2252-carrier-free

Carrier Free

$370

More conjugates/formats

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PA2252
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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