Anti-PIP5K2B PIP4K2B Antibody

PIP5K2B antibody

Boster Bio Anti-PIP5K2B PIP4K2B Antibody (Catalog # A09390). Tested in WB applications. This antibody reacts with Human, Mouse, Rat, Chicken, Primate.

Product Info Summary

SKU: A09390
Size: 100ug
Reactive Species: Chicken, Human, Mouse, Rat, Primate
Host: Rabbit
Application: WB

Product Name

Anti-PIP5K2B PIP4K2B Antibody

View all PIP5K2B Antibodies

SKU/Catalog Number

A09390

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-PIP5K2B PIP4K2B Antibody (Catalog # A09390). Tested in WB applications. This antibody reacts with Human, Mouse, Rat, Chicken, Primate.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is six (6) months from date of opening.

Cite This Product

Anti-PIP5K2B PIP4K2B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09390)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 50% (v/v) Glycerol

Clonality

Polyclonal

Isotype

IgG

Immunogen

PIP5K2B affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of human PIP5K2B.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A09390 is reactive to PIP4K2B in Chicken, Human, Mouse, Rat, Primate

Applications

A09390 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

47.378kDa

Background of PIP5K2B

PIP5K2B (phosphatidylinositol-4-phosphate 5-kinase, type II, beta) functions in the Pkc1p protein kinase pathway and is required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization. Anti-PIP5K2B antibodies are ideal for researchers interested in Cancer research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:20,000 - 1:60,000
IHC: 1:100-1:500
IF Microscopy: 1:100-1:500
WB: 0.2 µg/ml

Validation Images & Assay Conditions

Gene/Protein Information For PIP4K2B (Source: Uniprot.org, NCBI)

Gene Name

PIP4K2B

Full Name

Phosphatidylinositol 5-phosphate 4-kinase type-2 beta

Weight

47.378kDa

Alternative Names

1-phosphatidylinositol-4-phosphate kinase; 1-phosphatidylinositol-5-phosphate 4-kinase 2-beta; Diphosphoinositide kinase 2-beta; EC 2.7.1; EC 2.7.1.149; phosphatidylinositol-4-phosphate 5-kinase, type II, beta; Phosphatidylinositol-5-phosphate 4-kinase type II beta; phosphatidylinositol-5-phosphate 4-kinase type-2 beta; phosphatidylinositol-5-phosphate 4-kinase, type II, beta; PI(5)P 4-kinase type II beta; PI5P4KB; PIP4KII-beta; PIP5K2B; PIP5KIIB; PIP5KIIbeta; PTDINS(4)P-5-kinase; PtdIns(5)P-4-kinase isoform 2-beta PIP4K2B PI5P4KB, PIP5K2B, PIP5KIIB, PIP5KIIbeta, PIP5P4KB phosphatidylinositol-5-phosphate 4-kinase type 2 beta phosphatidylinositol 5-phosphate 4-kinase type-2 beta|1-phosphatidylinositol 5-phosphate 4-kinase 2-beta|1-phosphatidylinositol-4-phosphate kinase|PI(5)P 4-kinase type II beta|PIP4KII-beta|PTDINS(4)P-5-kinase|diphosphoinositide kinase 2-beta|phosphatidylinositol 5-phosphate 4-kinase type II beta|phosphatidylinositol-4-phosphate 5-kinase, type II, beta

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PIP4K2B, check out the PIP4K2B Infographic

PIP4K2B infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PIP4K2B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-PIP5K2B PIP4K2B Antibody

Question

See attached the WB image, lot number and protocol we used for cervix carcinoma using anti-PIP5K2B antibody A09390. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-08-28

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-28

Question

Does anti-PIP5K2B antibody A09390 work on horse WB with cerebral cortex?

Verified Customer

Verified customer

Asked: 2019-08-26

Answer

Our lab technicians have not tested anti-PIP5K2B antibody A09390 on horse. You can run a BLAST between horse and the immunogen sequence of anti-PIP5K2B antibody A09390 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse cerebral cortex in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-26

Question

We are currently using anti-PIP5K2B antibody A09390 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?

D. Krishna

Verified customer

Asked: 2019-05-01

Answer

The anti-PIP5K2B antibody (A09390) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-01

Question

My question regarding product A09390, anti-PIP5K2B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-03-01

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09390 anti-PIP5K2B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-03-01

Question

Is a blocking peptide available for product anti-PIP5K2B antibody (A09390)?

Verified Customer

Verified customer

Asked: 2018-01-19

Answer

We do provide the blocking peptide for product anti-PIP5K2B antibody (A09390). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-01-19

Order DetailsPrice
A09390

100 μg

$599

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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