Anti-PLK2 Antibody

PLK2 antibody

Boster Bio Anti-PLK2 Antibody catalog # PA2131. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA2131
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IHC, IHC-F, ICC, WB

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Product Name

Anti-PLK2 Antibody

View all PLK2 Antibodies

SKU/Catalog Number

PA2131

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PLK2 Antibody catalog # PA2131. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PLK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2131)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human PLK2, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2131 is reactive to PLK2 in Human, Mouse, Rat

Applications

PA2131 is guaranteed for Flow Cytometry, IHC, IHC-F, ICC, WB Boster Guarantee

Observed Molecular Weight

78 kDa

Calculated molecular weight

78.237kDa

Background of PLK2

Polo-like kinase2, also known as SNK is an enzyme that in humans is encoded by the PLK2 gene. PLK2 is a member of the "polo" family of serine/threonine protein kinases that have a role in normal cell division. The International Radiation Hybrid Mapping Consortium mapped the SNK gene to chromosome 5. SNK involved in synaptic plasticity, centriole duplication and G1/S phase transition. This gene plays a key role in synaptic plasticity and memory by regulating the Ras and Rap protein signalin: required for overactivity-dependent spine remodeling by phosphorylating the Ras activator RASGRF1 and the Rap inhibitor SIPA1L1 leading to their degradation by the proteasome.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For PLK2 (Source: Uniprot.org, NCBI)

Gene Name

PLK2

Full Name

Serine/threonine-protein kinase PLK2

Weight

78.237kDa

Superfamily

protein kinase superfamily

Alternative Names

EC 2.7.11; PLK-2; polo-like kinase 2EC 2.7.11.21; serine/threonine-protein kinase PLK2; Serine/threonine-protein kinase SNK; Serum-inducible kinase; SNKpolo-like kinase 2 (Drosophila) PLK2 SNK, hPlk2, hSNK polo like kinase 2 serine/threonine-protein kinase PLK2|PLK-2|serine/threonine-protein kinase SNK|serum-inducible kinase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PLK2, check out the PLK2 Infographic

PLK2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PLK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-PLK2 Antibody

Question

I was wanting to use your anti-PLK2 antibody for Flow Cytometry for human colon on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human colon identification?

Verified Customer

Verified customer

Asked: 2020-03-10

Answer

It shows on the product datasheet, PA2131 anti-PLK2 antibody has been tested for Flow Cytometry, IHC-P, IHC-F, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human colon in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-03-10

Question

I have a question about product PA2131, anti-PLK2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Z. Thomas

Verified customer

Asked: 2020-02-25

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2131 anti-PLK2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-02-25

Question

Will anti-PLK2 antibody PA2131 work on horse IHC-P with frontal cortex?

L. Anderson

Verified customer

Asked: 2020-01-16

Answer

Our lab technicians have not validated anti-PLK2 antibody PA2131 on horse. You can run a BLAST between horse and the immunogen sequence of anti-PLK2 antibody PA2131 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse frontal cortex in IHC-P, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-16

Question

We are currently using anti-PLK2 antibody PA2131 for rat tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-24

Answer

The anti-PLK2 antibody (PA2131) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-24

Question

Do you have a BSA free version of anti-PLK2 antibody PA2131 available?

J. Brown

Verified customer

Asked: 2013-07-23

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PLK2 antibody PA2131 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2013-07-23

Order DetailsPrice
PA2131

100μg

$370
PA2131-10ug

10μg sample (liquid)

$99
PA2131-Biotin

100 μg Biotin conjugated

$570
PA2131-Cy3

100 μg Cy3 conjugated

$570
PA2131-Dylight488

100 μg Dylight488 conjugated

$570
PA2131-Dylight550

100 μg Dylight550 conjugated

$570
PA2131-Dylight594

100 μg Dylight594 conjugated

$570
PA2131-FITC

100 μg FITC conjugated

$570
PA2131-HRP

100 μg HRP conjugated

$570
PA2131-APC

100 μg APC conjugated

$670
PA2131-PE

100 μg PE conjugated

$670
PA2131-carrier-free

Carrier Free

$370

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PA2131
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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