|Application:||IHC-P, ICC, WB|
Data & Images
|Product Name||Anti-PLK2 Antibody|
|Description||Rabbit IgG polyclonal antibody for Serine/threonine-protein kinase PLK2(PLK2) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.|
|Cite This Product||Anti-PLK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2131)|
|Replacement Item||This antibody may replace the following items: sc-32250|sc-32246|sc-34843|sc-34842|sc-20731|sc-373983|sc-34840|sc-1410|sc-373984|sc-365826|sc-101501|sc-514019 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
|Application||IHC-P, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human PLK2(666-685aa SSELKNRMEYALNMLLQRCN), different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Serine/threonine-protein kinase PLK2|
|Molecular Weight||78237 MW|
|Protein Function||Tumor suppressor serine/threonine-protein kinase involved in synaptic plasticity, centriole duplication and G1/S phase transition. Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates CENPJ, NPM1, RAPGEF2, RASGRF1, SNCA, SIPA1L1 and SYNGAP1. Plays a key role in synaptic plasticity and memory by regulating the Ras and Rap protein signaling: required for overactivity-dependent spine remodeling by phosphorylating the Ras activator RASGRF1 and the Rap inhibitor SIPA1L1 leading to their degradation by the proteasome. Conversely, phosphorylates the Rap activator RAPGEF2 and the Ras inhibitor SYNGAP1, promoting their activity. Also regulates synaptic plasticity independently of kinase activity, via its interaction with NSF that disrupts the interaction between NSF and the GRIA2 subunit of AMPARs, leading to a rapid rundown of AMPAR-mediated current that occludes long term depression. Required for procentriole formation and centriole duplication by phosphorylating CENPJ and NPM1, respectively. Its induction by p53/TP53 suggests that it may participate in the mitotic checkpoint following stress. .|
|Tissue Specificity||Expressed at higher level in the fetal lung, kidney, spleen and heart. .|
|Sequence Similarities||Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.|
|Subcellular Localization||Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Cell projection, dendrite . Localizes to centrosomes during early G1 phase where it only associates to the mother centriole and then distributes equally to both mother and daughter centrioles at the onset of S phase.|
|Alternative Names||Serine/threonine-protein kinase PLK2;184.108.40.206;Polo-like kinase 2;PLK-2;hPlk2;Serine/threonine-protein kinase SNK;hSNK;Serum-inducible kinase;PLK2;SNK;|
|Research Areas|||signal transduction|protein phosphorylation|ser / thr kinases|other kinases||
Background for Serine/threonine-protein kinase PLK2
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-PLK2 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-PLK2 Antibody Images
Click the images to enlarge.
Lane 1: A431 Cell Lysate
Lane 2: 293T Cell Lysate
Lane 3: COLO320 Cell Lysate
IHC(P): Human Lung Cancer Tissue
IHC(P): Rat Intestine Tissue
IHC(P): Rat Brain Tissue
- Review by Wei Zou
"Test of PLK2 in S1 and 293 Cells"
--Wei Zou, MICROBIOLOGY, UNIVERSITY OF KANSAS MEDICAL CENTER, POSTDOC
Applications Western Blot Sample Cell lysis sample Detection ECL method
"I wanted to investigate the expression of PLK2 protein after ShRNA-Plk2 treatment in UT7/Epo-S1 cell. Western blot was applied to test the expression of PLK2 with the antibody from Boster (PA2131, small volume). I chose this antibody because of the small volume and it claimed to work on western blot. A lot of unspecific bands...This antibody may not be good for western blot in UT7/Epo-S1 cell and 293 cells."
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,