Anti-Podoplanin/gp36/PDPN Antibody

Podoplanin antibody

Boster Bio Anti-Podoplanin/gp36/PDPN Antibody catalog # PA1675. Tested in WB applications. This antibody reacts with Mouse, Rat.

Product Info Summary

SKU: PA1675
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-Podoplanin/gp36/PDPN Antibody

View all Podoplanin Antibodies

SKU/Catalog Number

PA1675

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Podoplanin/gp36/PDPN Antibody catalog # PA1675. Tested in WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Podoplanin/gp36/PDPN Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1675)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of rat Podoplanin/gp36, different from the related mouse sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1675 is reactive to PDPN in Mouse, Rat

Applications

PA1675 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

38 kDa

Calculated molecular weight

16.698kDa

Background of Podoplanin

PDPN (Podoplanin), also called T1A, T1A2, GP36, OTS8 or AGGRUS, is a protein that in humans is encoded by the PDPN gene. This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The PDPN gene is mapped to chromosome 1 by the International radiation Hybrid mapping consortium. The physiological function of PDPN may be related to its mucin-type character. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Immunohistochemical analysis of PDPN in placenta, kidney, lung, and nasal polyps showed expression at the apical plasma membrane of vascular endothelial cells and in alveolar epithelial cells. Overexpression of rat PDPN in human and rodent endothelial cells promoted formation of elongated cell extensions and significantly increased endothelial cell adhesion, migration, and tube formation. Inhibition of PDPN expression by small interfering RNAs decreased cell adhesion in cultured human dermal lymphatic endothelial cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For PDPN (Source: Uniprot.org, NCBI)

Gene Name

PDPN

Full Name

Podoplanin

Weight

16.698kDa

Superfamily

podoplanin family

Alternative Names

36-KD; Aggrus; Gp36; Gp38; GP40; HT1A-1; hT1alpha-1; hT1alpha-2; lung type I cell membrane associated glycoprotein; lung type-I cell membrane-associated glycoprotein (T1A-2); OTS 8; OTS8; PA2.26 antigen; PDPN; Podoplanin; RANDAM-2; T1A; T1A-2; T1-alpha PDPN AGGRUS, GP36, GP40, Gp38, HT1A-1, OTS8, PA2.26, T1A, T1A-2, T1A2, TI1A podoplanin podoplanin|PA2.26 antigen|T1-alpha|glycoprotein 36|glycoprotein, 36-KD|hT1alpha-1|hT1alpha-2|lung type I cell membrane associated glycoprotein|lung type-I cell membrane-associated glycoprotein (T1A-2)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PDPN, check out the PDPN Infographic

PDPN infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PDPN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-Podoplanin/gp36/PDPN Antibody

Question

We are currently using anti-Podoplanin/gp36/PDPN antibody PA1675 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-11

Answer

The anti-Podoplanin/gp36/PDPN antibody (PA1675) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-11

Question

Do you have a BSA free version of anti-Podoplanin/gp36/PDPN antibody PA1675 available?

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Podoplanin/gp36/PDPN antibody PA1675 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-23

Question

We need to test anti-Podoplanin/gp36/PDPN antibody PA1675 on rat brain for research purposes, then I may be interested in using anti-Podoplanin/gp36/PDPN antibody PA1675 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-10-23

Answer

The products we sell, including anti-Podoplanin/gp36/PDPN antibody PA1675, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-10-23

Question

Does anti-Podoplanin/gp36/PDPN antibody PA1675 work on bovine WB with placenta {eco:0000312?

Verified Customer

Verified customer

Asked: 2019-08-28

Answer

Our lab technicians have not tested anti-Podoplanin/gp36/PDPN antibody PA1675 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Podoplanin/gp36/PDPN antibody PA1675 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine placenta {eco:0000312 in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-28

Question

I see that the anti-Podoplanin/gp36/PDPN antibody PA1675 works with WB, what is the protocol used to produce the result images on the product page?

V. Jones

Verified customer

Asked: 2018-01-15

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-01-15

Question

I was wanting to use your anti-Podoplanin/gp36/PDPN antibody for WB for rat brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat brain identification?

Verified Customer

Verified customer

Asked: 2017-08-24

Answer

You can see on the product datasheet, PA1675 anti-Podoplanin/gp36/PDPN antibody has been validated for WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-08-24

Question

Does PA1675 anti-Podoplanin/gp36/PDPN antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

W. Wu

Verified customer

Asked: 2014-02-12

Answer

As indicated on the product datasheet, PA1675 anti-Podoplanin/gp36/PDPN antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-02-12

Order DetailsPrice
PA1675

100μg

$370
PA1675-10ug

10μg sample (liquid)

$99
PA1675-Biotin

100 μg Biotin conjugated

$570
PA1675-Cy3

100 μg Cy3 conjugated

$570
PA1675-Dylight488

100 μg Dylight488 conjugated

$570
PA1675-Dylight550

100 μg Dylight550 conjugated

$570
PA1675-Dylight594

100 μg Dylight594 conjugated

$570
PA1675-FITC

100 μg FITC conjugated

$570
PA1675-HRP

100 μg HRP conjugated

$570
PA1675-APC

100 μg APC conjugated

$670
PA1675-PE

100 μg PE conjugated

$670
PA1675-carrier-free

Carrier Free

$370

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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