Data & Images
|Product Name||Anti-PON1/Paraoxonase 1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Serum paraoxonase/arylesterase 1(PON1) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-PON1/Paraoxonase 1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2253)|
|Replacement Item||This antibody may replace the following items: sc-21146|sc-133919|sc-21147|sc-59646 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human PON1(186-201aa FLDPYLQSWEMYLGLA), different from the related mouse and rat sequences by three amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Serum paraoxonase/arylesterase 1|
|Molecular Weight||39731 MW|
|Protein Function||Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. .|
|Tissue Specificity||Plasma, associated with HDL (at protein level). Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas. .|
|Sequence Similarities||Belongs to the paraoxonase family.|
|Subcellular Localization||Secreted, extracellular space.|
|Alternative Names||Serum paraoxonase/arylesterase 1;PON 1;126.96.36.199;188.8.131.52;184.108.40.206;Aromatic esterase 1;A-esterase 1;K-45;Serum aryldialkylphosphatase 1;PON1;PON;|
|Research Areas|||neuroscience|neurology process|neurodegenerative disease|parkinson's disease| cardiovascular|lipids / lipoproteins|lipid metabolism|hydrolysis|lipoproteins/apolipoproteins| neuroscience| signal transduction|drug metabolism| cell biology|other antibodies|oxidative stress|atherosclerosis|diabetes associated| metabolism|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|redox metabolism|types of disease|diabetes|cancer|heart disease||
Background for Serum paraoxonase/arylesterase 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-PON1/Paraoxonase 1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-PON1/Paraoxonase 1 Antibody Images
Click the images to enlarge.
All lanes: Anti-PON1(PA2253) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 40ug
Lane 2: Rat Lung Tissue Lysate at 40ug
Lane 3: Human Placenta Tissue Lysate at 40ug
Lane 4: Rat Testis Tissue Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: HEPA Whole Cell Lysate at 40ug
Lane 7: A549 Whole Cell Lysate at 40ug
Lane 8: JURKAT Whole Cell Lysate at 40ug
Lane 9: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 40KD
Observed bind size: 40KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,