Anti-PON2 Antibody Picoband™

PON2 antibody

Boster Bio Anti-PON2 Antibody Picoband™ catalog # PB9779. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: PB9779
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-PON2 Antibody Picoband™

View all PON2 Antibodies

SKU/Catalog Number

PB9779

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PON2 Antibody Picoband™ catalog # PB9779. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PON2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9779)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human PON2, different from the related mouse and rat sequences by four amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9779 is reactive to PON2 in Human

Applications

PB9779 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

39.381kDa

Background of PON2

PON2 is an enzyme that in humans is encoded by the PON2 gene. It is mapped to 7q21.3. This gene encodes a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones and important bacterial quorum-sensing mediators suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For PON2 (Source: Uniprot.org, NCBI)

Gene Name

PON2

Full Name

Serum paraoxonase/arylesterase 2

Weight

39.381kDa

Superfamily

paraoxonase family

Alternative Names

A-esterase 2; Aromatic esterase 2; EC 3.1.1.2; EC 3.1.1.81; paraoxonase 2; paraoxonase nirs; PON 2; PON2; Serum Aryldialkylphosphatase 2; serum paraoxonase/arylesterase 2 PON2 paraoxonase 2 serum paraoxonase/arylesterase 2|A-esterase 2|PON 2|aromatic esterase 2|arylesterase 2|paraoxonase nirs|serum aryldialkylphosphatase 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PON2, check out the PON2 Infographic

PON2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PON2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-PON2 Antibody Picoband™

Question

We are currently using anti-PON2 antibody PB9779 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-13

Answer

The anti-PON2 antibody (PB9779) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-13

Question

Will anti-PON2 antibody PB9779 work on goat WB with skin?

Verified Customer

Verified customer

Asked: 2019-10-30

Answer

Our lab technicians have not tested anti-PON2 antibody PB9779 on goat. You can run a BLAST between goat and the immunogen sequence of anti-PON2 antibody PB9779 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat skin in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-30

Question

Does PB9779 anti-PON2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

E. Carter

Verified customer

Asked: 2018-08-29

Answer

You can see on the product datasheet, PB9779 anti-PON2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-08-29

Question

My question regarding product PB9779, anti-PON2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-10-10

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9779 anti-PON2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-10-10

Question

Is there a BSA free version of anti-PON2 antibody PB9779 available?

M. Li

Verified customer

Asked: 2014-09-15

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PON2 antibody PB9779 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2014-09-15

Order DetailsPrice
PB9779

100μg

$370
PB9779-10ug

10μg sample (liquid)

$99
PB9779-Biotin

100 μg Biotin conjugated

$570
PB9779-Cy3

100 μg Cy3 conjugated

$570
PB9779-Dylight488

100 μg Dylight488 conjugated

$570
PB9779-Dylight550

100 μg Dylight550 conjugated

$570
PB9779-Dylight594

100 μg Dylight594 conjugated

$570
PB9779-FITC

100 μg FITC conjugated

$570
PB9779-HRP

100 μg HRP conjugated

$570
PB9779-APC

100 μg APC conjugated

$670
PB9779-PE

100 μg PE conjugated

$670
PB9779-carrier-free

Carrier Free

$370

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PB9779
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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