Product Info Summary
SKU: | A01893T301 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-PP2A-Alpha (T301) PPP2CA Antibody
View all PP2A alpha Antibodies
SKU/Catalog Number
A01893T301
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PP2A-Alpha (T301) PPP2CA Antibody catalog # A01893T301. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PP2A-Alpha (T301) PPP2CA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01893T301)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human MDMX around the phosphorylation site of S367.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01893T301 is reactive to PPP2CA in Human, Mouse, Rat
Applications
A01893T301 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
35.594kDa
Background of PP2A alpha
Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway.
McKay M.J., Genomics 36:305-315(1996).
Sadano H., Biochem. Biophys. Res. Commun. 267:418-422(2000).
Nomura N., DNA Res. 1:223-229(1994).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of PPP2CA using Anti-PP2A-Alpha (T301) PPP2CA Antibody (A01893T301).
Immunohistochemistry (IHC) analyzes of PP2A-α (T301) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-PP2A-Alpha (T301) PPP2CA Antibody A01893T301
Western blot (WB) analysis of PP2A-α (T301) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate(40ug)Lane2:COS-7 whole cell lysate(40ug)
Lane3:C6 whole cell lysate(40ug)
Lane4:NIH-3T3 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For PPP2CA (Source: Uniprot.org, NCBI)
Gene Name
PPP2CA
Full Name
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
Weight
35.594kDa
Superfamily
PPP phosphatase family
Alternative Names
EC 3.1.3.16; PP2A; PP2A-alpha; PP2Ac; PP2CA; PP2Calpha; PPP2CA; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isozyme; protein phosphatase 2A catalytic subunit, alpha isoform; Replication protein C; RP-C; serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform; serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform PPP2CA NEDLBA, PP2Ac, PP2CA, PP2Calpha, RP-C protein phosphatase 2 catalytic subunit alpha serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform|PP2A-alpha|protein phosphatase 2, catalytic subunit, alpha isozyme|replication protein C
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPP2CA, check out the PPP2CA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPP2CA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PP2A-Alpha (T301) PPP2CA Antibody (A01893T301)
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No publications found for A01893T301
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