Product Info Summary
SKU: | M04723 |
---|---|
Size: | 100ug |
Reactive Species: | Human |
Host: | Mouse |
Application: | IF, ICC, WB |
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Product info
Product Name
Anti-PP5 Monoclonal Antibody
SKU/Catalog Number
M04723
Size
100ug
Form
liquid
Description
Boster Bio Anti-PP5 Monoclonal Antibody catalog # M04723. Tested in IF, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PP5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04723)
Host
Mouse
Contents
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Clonality
Monoclonal
Clone Number
2E11
Isotype
IgG2a
Immunogen
Full length human PP5 protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~58kDa.
Reactive Species
M04723 is reactive to PPP5C in Human
Applications
M04723 is guaranteed for IF, ICC, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
56.879kDa
Background of PP5
Proteins in this family participate in pathways regulated by reversible phosphorylation at serine and threonine residues, potentially playing a role in the regulation of cell growth and differentiation. PP5 or PPT is predominantly a nuclear protein and interacts with CDC16 and CDC27. It is found in association with several proteins that influence intracellular signlaing-cascades intitiated by hormones (glucocorticoids) or cellular stress (hypoxia, oxidative stress and DNA Damage). It phosphorylates serine residues of skeletal muscle phosphorylase and histone H1. It may also be involved in mitosis and RNA biogenesis regulation (1,2).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Western blot analysis of PPP5C using anti-PPP5C antibody (M04723).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP5C antigen affinity purified polyclonal antibody (Catalog # M04723) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for PPP5C.
Click image to see more details
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PP5 Monoclonal Antibody, Clone 2E11 (M04723) . Tissue: Cervical Cancer cell line (HeLa) . Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody (M04723) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 40X.
Click image to see more details
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PP5 Monoclonal Antibody, Clone 2E11 (M04723) . Tissue: HEK293. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody (M04723) at 1:50 for 1 hour at RT. Secondary Antibody: Alexa Fluor 488 Goat Anti-Mouse (green) at 1:100 for 1 hour at RT. Counterstain: DAPI (blue) nuclear stain. Magnification: 63x.
Protein Target Info & Infographic
Gene/Protein Information For PPP5C (Source: Uniprot.org, NCBI)
Gene Name
PPP5C
Full Name
Serine/threonine-protein phosphatase 5
Weight
56.879kDa
Superfamily
PPP phosphatase family
Alternative Names
EC 3.1.3.16; PP5; PP5FLJ36922; PPP5; PPP5C; PPT; PP-T; protein phosphatase 5, catalytic subunit; Protein phosphatase T; Serine/Threonine phosphatase; serine/threonine-protein phosphatase 5 PPP5C PP5, PPP5, PPT protein phosphatase 5 catalytic subunit serine/threonine-protein phosphatase 5|PP-T|protein phosphatase T
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPP5C, check out the PPP5C Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPP5C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PP5 Monoclonal Antibody (M04723)
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No publications found for M04723
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-PP5 Monoclonal Antibody
Question
We are currently using anti-PP5 Monoclonal antibody M04723 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
The anti-PP5 Monoclonal antibody (M04723) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-28