|Application:||IHC-P, IHC-F, ICC, WB|
Data & Images
|Product Name||Anti-PPAR Alpha Antibody|
|Description||Rabbit IgG polyclonal antibody for Peroxisome proliferator-activated receptor alpha(PPARA) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.|
|Cite This Product||Anti-PPAR Alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1412)|
|Replacement Item||This antibody may replace the following items: sc-130640|sc-1982|sc-1982-X|sc-1985|sc-1985-X|sc-398394|sc-398394-X|sc-9000|sc-9000-X from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Application||IHC-P, IHC-F, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of mouse PPAR alpha(393-412aa NIGYIEKLQEGIVHVLKLHL), different from the human sequence by four amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Peroxisome proliferator-activated receptor alpha(PPAR-alpha)|
|Molecular Weight||52347 MW|
|Protein Function||Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl- 2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2. .|
|Tissue Specificity||Highly expressed in liver, kidney and heart. Very weakly expressed in brain and testis.|
|Sequence Similarities||Belongs to the nuclear hormone receptor family. NR1 subfamily.|
|Subcellular Localization||Nucleus .|
|Alternative Names||Peroxisome proliferator-activated receptor alpha;PPAR-alpha;Nuclear receptor subfamily 1 group C member 1;Ppara;Nr1c1, Ppar;|
|Research Areas|||epigenetics and nuclear signaling|nuclear signaling pathways|nuclear receptors|co-activators/co-repressors| epigenetics and nuclear signaling|transcription|other factors| cardiovascular|atherosclerosis|diabetes associated|lipids / lipoproteins|fatty acids|metabolism| metabolism|pathways and processes|mitochondrial metabolism|mitochondrial biogenesis|metabolic signaling pathways|nucleotide metabolism|molecular processes|mitochondrial transcription|lipid and lipoprotein metabolism|types of disease|diabetes|obesity|cancer|heart disease|metabolic disorders|redox metabolism|fatty acid oxidation||
Background for Peroxisome proliferator-activated receptor alpha(PPAR-alpha)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-PPAR Alpha Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human, Mouse, Rat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-PPAR Alpha Antibody Images
Click the images to enlarge.
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Cardiac Muscle Tissue Lysate
Lane 4: MM231 Cell Lysate
Lane 5: HELA Cell Lysate
Lane 6: SMMC Cell Lysate
Lane 7: HT1080 Cell Lysate
Lane 8: SW620 Cell Lysate
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
IHC(P): Mouse Intestine Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,