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SKU:PA1412
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC-P, IHC-F, ICC, WB
Price: $200.00
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Overview

Product Name Anti-PPAR Alpha Antibody
SKU/Catalog Number PA1412
Description Rabbit IgG polyclonal antibody for Peroxisome proliferator-activated receptor alpha(PPARA) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.
Cite This Product Anti-PPAR Alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1412)
Replacement Item This antibody may replace the following items: sc-130640|sc-1982|sc-1982-X|sc-1985|sc-1985-X|sc-398394|sc-398394-X|sc-9000|sc-9000-X from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, IHC-F, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of mouse PPAR alpha(393-412aa NIGYIEKLQEGIVHVLKLHL), different from the human sequence by four amino acids.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Ppara
Protein Name Peroxisome proliferator-activated receptor alpha(PPAR-alpha)
Molecular Weight 52347 MW
Protein Function Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl- 2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2. .
Tissue Specificity Highly expressed in liver, kidney and heart. Very weakly expressed in brain and testis.
Sequence Similarities Belongs to the nuclear hormone receptor family. NR1 subfamily.
Subcellular Localization Nucleus .
Uniprot ID P23204
Alternative Names Peroxisome proliferator-activated receptor alpha;PPAR-alpha;Nuclear receptor subfamily 1 group C member 1;Ppara;Nr1c1, Ppar;
Research Areas |epigenetics and nuclear signaling|nuclear signaling pathways|nuclear receptors|co-activators/co-repressors| epigenetics and nuclear signaling|transcription|other factors| cardiovascular|atherosclerosis|diabetes associated|lipids / lipoproteins|fatty acids|metabolism| metabolism|pathways and processes|mitochondrial metabolism|mitochondrial biogenesis|metabolic signaling pathways|nucleotide metabolism|molecular processes|mitochondrial transcription|lipid and lipoprotein metabolism|types of disease|diabetes|obesity|cancer|heart disease|metabolic disorders|redox metabolism|fatty acid oxidation|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Peroxisome proliferator-activated receptor alpha(PPAR-alpha)

Peroxisome proliferator-activated receptor alpha(PPAR-alpha), also known as NR1C1(nuclear receptor subfamily 1, group C, member 1), is a nuclear receptor protein that in humans is encoded by the PPARA gene. PPARA gene spans 83.7 kb and contains 8 exons. And the PPAR gene is mapped to chromosome 22q12-q13.1. Sher et al.(1993) cloned a cDNA for human peroxisome proliferator-activated receptor from a human liver cDNA library. The PPAR cDNA exhibited 85% and 91% DNA and deduced amino acid sequence identity, respectively, with mouse PPAR. PPAR-alpha is a transcription factor and a major regulator of lipid metabolism in the liver.

Anti-PPAR Alpha Antibody Images

Click the images to enlarge.

Anti-PPAR Alpha Antibody
Anti-PPAR alpha antibody, PA1412, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Cardiac Muscle Tissue Lysate
Lane 4: MM231 Cell Lysate
Lane 5: HELA Cell Lysate
Lane 6: SMMC Cell Lysate
Lane 7: HT1080 Cell Lysate
Lane 8: SW620 Cell Lysate
Anti-PPAR Alpha Antibody
Anti-PPAR alpha antibody, PA1412, IHC(P)
IHC(P): Human Mammary Cancer Tissue

Anti-PPAR Alpha Antibody
Anti-PPAR alpha antibody, PA1412, IHC(P)
IHC(P): Human Mammary Cancer Tissue

Anti-PPAR Alpha Antibody
Anti-PPAR alpha antibody, PA1412, IHC(P)
IHC(P): Mouse Intestine Tissue
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Publications

Shi P, Zhang Xx, Zhang Z, Zhang Y, Wu B, Cheng S, Li A. Chemosphere. 2012 Jul;88(4):407-12. Doi: 10.1016/J.Chemosphere.2012.02.055. Epub 2012 Mar 24. Chronic Exposure To Contaminated Drinking Water Stimulates Ppar Expression In Mice Livers.
Li Jp, Chen S, Peng H, Zhou Jl, Fang Hs. Bioelectromagnetics. 2014 Apr;35(3):170-80. Doi: 10.1002/Bem.21833. Epub 2014 Jan 14. Pulsed Electromagnetic Fields Protect The Balance Between Adipogenesis And Osteogenesis On Steroid-Induced Osteonecrosis...
Zhou X, Zhang J, Xu C, Wang W. J Pharmacol Sci. 2014;126(4):344-50. Doi: 10.1254/Jphs.14173Fp. Epub 2014 Dec 2. Curcumin Ameliorates Renal Fibrosis By Inhibiting Local Fibroblast Proliferation And Extracellular Matrix Deposition.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.