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Product Info Summary
| SKU: | A04841 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PRKAG3 Antibody (C-term)
SKU/Catalog Number
A04841
Size
80 µl
Description
Boster Bio Anti-PRKAG3 Antibody (C-term) (Catalog # A04841). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PRKAG3 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04841)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This PRKAG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 426-454 amino acids from the C-terminal region of human PRKAG3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04841 is reactive to PRKAG3 in Human
Reconstitution
Calculated molecular weight
54258 Da
Background of PRKAG3
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. It is dominantly expressed in skeletal muscle. Studies of the pig counterpart suggest that this subunit may play a key role in the regulation of energy metabolism in skeletal muscle. [provided by RefSeq].
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04841 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
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Western blot analysis of lysates from Hela, HepG2, RD cell line (from left to right), using PRKAG3 Antibody (C-term). A04841 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
Protein Target Info & Infographic
Gene/Protein Information For PRKAG3 (Source: Uniprot.org, NCBI)
Gene Name
PRKAG3
Full Name
5'-AMP-activated protein kinase subunit gamma-3
Weight
54258 Da
Superfamily
5'-AMP-activated protein kinase gamma subunit family
Alternative Names
5'-AMP-activated protein kinase subunit gamma-3, AMPK gamma3, AMPK subunit gamma-3, PRKAG3, AMPKG3 PRKAG3 AMPKG3, SMGMQTL protein kinase AMP-activated non-catalytic subunit gamma 3 5-AMP-activated protein kinase subunit gamma-3|5-AMP-activated protein kinase, gamma-3 subunit|AMPK gamma-3 chain|AMPK gamma3|AMPK subunit gamma-3|protein kinase, AMP-activated, gamma 3 non-catalytic subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKAG3, check out the PRKAG3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKAG3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PRKAG3 Antibody (C-term) (A04841)
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