Anti-Procalcitonin (4C8.H6.D4) Calca Monoclonal Antibody

CALCA antibody

Boster Bio Anti-Procalcitonin (4C8.H6.D4) Calca Monoclonal Antibody (Catalog # M02352). Tested in ELISA, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: M02352
Size: 100ug
Reactive Species: Human
Host: Mouse
Application: ELISA, WB

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Product Name

Anti-Procalcitonin (4C8.H6.D4) Calca Monoclonal Antibody

View all CALCA Antibodies

SKU/Catalog Number

M02352

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Procalcitonin (4C8.H6.D4) Calca Monoclonal Antibody (Catalog # M02352). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Procalcitonin (4C8.H6.D4) Calca Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02352)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 4C8.H6.D4 IgG1 lambda

Isotype

IgG1 lambda

Immunogen

Procalcitonin Antibody was produced in mice prepared by repeated immunizations with full-length recombinant human Procalcitonin protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

M02352 is reactive to CALCA in Human

Applications

M02352 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

15.467kDa

Background of CALCA

Anti-Procalcitonin antibody detects human Procalcitonin. Procalcitonin is a peptide hormone mainly produced by the C cells of the thyroid and certain endocrine cells of the lung. Under normal expression conditions, procalcitonin is immediately cleaved into three specific fragments, a N terminal residue, calcitonin and katacalcin. Levels of unprocessed procalcitonin rise significantly after bacterial infection, trauma or shock. This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: User optimized
WB: 1:1,000 - 1:2,000

Validation Images & Assay Conditions

Gene/Protein Information For CALCA (Source: Uniprot.org, NCBI)

Gene Name

CALCA

Full Name

Calcitonin

Weight

15.467kDa

Superfamily

calcitonin family

Alternative Names

CALC1; CALCA; Calcitonin 1; calcitonin gene-related peptide 1; CGRP; Katacalcin; Procalcitonin CALCA CALC1, CGRP, CGRP-I, CGRP-alpha, CGRP1, CT, KC, PCT calcitonin related polypeptide alpha calcitonin|calcitonin gene-related peptide 1|alpha-type CGRP|calcitonin 1|calcitonin gene-related peptide I|calcitonin/calcitonin-related polypeptide, alpha|katacalcin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CALCA, check out the CALCA Infographic

CALCA infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CALCA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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13 Customer Q&As for Anti-Procalcitonin (4C8.H6.D4) Calca Monoclonal Antibody

Question

I was wanting to use your anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody for WB for human pheochromocytoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human pheochromocytoma identification?

Verified Customer

Verified customer

Asked: 2020-02-19

Answer

As indicated on the product datasheet, M02352 anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human pheochromocytoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-19

Question

Is a blocking peptide available for product anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody (M02352)?

Verified Customer

Verified customer

Asked: 2019-12-31

Answer

We do provide the blocking peptide for product anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody (M02352). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-12-31

Question

Is there a BSA free version of anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody M02352 available?

Verified Customer

Verified customer

Asked: 2019-12-24

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody M02352 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-24

Question

My colleagues were satisfied with the WB result of your anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody. However we have been able to see positive staining in dorsal root ganglion secreted. using this antibody. Is that expected? Could you tell me where is CALCA supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-11-08

Answer

According to literature, dorsal root ganglion does express CALCA. Generally CALCA expresses in secreted. Regarding which tissues have CALCA expression, here are a few articles citing expression in various tissues:
Pheochromocytoma, Pubmed ID: 1318039
Thyroid carcinoma, Pubmed ID: 6610687

Boster Scientific Support

Answered: 2019-11-08

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pheochromocytoma using anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody M02352. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-06-17

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-17

Question

We are currently using anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody M02352 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

The anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody (M02352) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-24

Question

Does M02352 anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-04-22

Answer

You can see on the product datasheet, M02352 anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-04-22

Question

I see that the anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody M02352 works with WB, what is the protocol used to produce the result images on the product page?

A. Mitchell

Verified customer

Asked: 2018-07-20

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-07-20

Question

I have a question about product M02352, anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

K. Taylor

Verified customer

Asked: 2015-04-23

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02352 anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2015-04-23

Question

Will anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody M02352 work for WB with pheochromocytoma?

J. Jones

Verified customer

Asked: 2014-02-11

Answer

According to the expression profile of pheochromocytoma, CALCA is highly expressed in pheochromocytoma. So, it is likely that anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody M02352 will work for WB with pheochromocytoma.

Boster Scientific Support

Answered: 2014-02-11

Question

Our lab want to know about to test anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody M02352 on human pheochromocytoma for research purposes, then I may be interested in using anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody M02352 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

J. Roberts

Verified customer

Asked: 2014-02-04

Answer

The products we sell, including anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody M02352, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2014-02-04

Question

See attached the WB image, lot number and protocol we used for pheochromocytoma using anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody M02352. Please let me know if you require anything else.

S. Jones

Verified customer

Asked: 2013-06-18

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-06-18

Question

We have observed staining in human pheochromocytoma. Do you have any suggestions? Is anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody supposed to stain pheochromocytoma positively?

D. Williams

Verified customer

Asked: 2013-03-12

Answer

From what I have seen in literature pheochromocytoma does express CALCA. From what I have seen in Uniprot.org, CALCA is expressed in dorsal root ganglion, thyroid carcinoma, pheochromocytoma, among other tissues. Regarding which tissues have CALCA expression, here are a few articles citing expression in various tissues:
Pheochromocytoma, Pubmed ID: 1318039
Thyroid carcinoma, Pubmed ID: 6610687

Boster Scientific Support

Answered: 2013-03-12

Order DetailsPrice
M02352-100uL

100uL

$612

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