Data & Images
|Product Name||Anti-Progesterone Receptor Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Progesterone receptor(PGR) detection. Tested with WB, IHC-P in Human;Rat.|
|Cite This Product||Anti-Progesterone Receptor Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9009)|
|Replacement Item||This antibody may replace the following items: sc-101786|sc-130071|sc-166169|sc-166169-X|sc-166170|sc-166170-X|sc-398898|sc-398898-X|sc-538|sc-538-X|sc-539|sc-539-X|sc-53943|sc-56862|sc-57553|sc-7208|sc-7208-X|sc-810|sc-810-X|sc-811|sc-811-X from Santa Cruz Biotechnology.|
|Validated Species||Human, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human Progesterone Receptor recombinant protein (Position: M595-K933). Human Progesterone Receptor shares 95% and 94% amino acid (aa) sequences identity with mouse and rat Progesterone Receptor, respectively.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Progesterone receptor|
|Molecular Weight||98981 MW|
|Protein Function||The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.|
|Sequence Similarities||Belongs to the nuclear hormone receptor family. NR3 subfamily.|
|Subcellular Localization||Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases.|
|Alternative Names||Progesterone receptor;PR;Nuclear receptor subfamily 3 group C member 3;PGR;NR3C3;|
|Research Areas|||epigenetics and nuclear signaling|transcription|domain families|zinc finger| signal transduction|signaling pathway|nuclear hormone receptors|progesterone| neuroscience|endocrine system|gonadotrophic axis| epigenetics and nuclear signaling|nuclear signaling pathways|nuclear receptors| cancer|tumor biomarkers||
Background for Progesterone receptor
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Progesterone Receptor Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Progesterone Receptor Picoband™ Antibody Images
Click the images to enlarge.
IHC(P): Human Mammary Cancer Tissue
All lanes: Anti-Progesterone Receptor(PB9009) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: SKOV Whole Cell Lysate at 40ug
Lane 4: Rat Brain Tissue Lysate at 40ug
Lane 5: Rat Testis Tissue Lysate at 40ug
Predicted bind size: 99KD
Observed bind size: 99KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,