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SKU:PA1583
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-Prostaglandin E Receptor EP1 Antibody
SKU/Catalog Number PA1583
Description Rabbit IgG polyclonal antibody for Prostaglandin E2 receptor EP1 subtype(PTGER1) detection. Tested with WB in Human.
Cite This Product Anti-Prostaglandin E Receptor EP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1583)
Replacement Item This antibody may replace the following items: sc-20631|sc-19552|sc-271022|sc-19554|sc-271023|sc-19551 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Prostaglandin E Receptor EP1(387-404aa AWEASSLRSSRHSGLSHF).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name PTGER1
Protein Name Prostaglandin E2 receptor EP1 subtype(PGE receptor EP1 subtype/PGE2 receptor EP1 subtype)
Molecular Weight 41801 MW
Protein Function Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(q) proteins which activate a phosphatidylinositol-calcium second messenger system. May play a role as an important modulator of renal function. Implicated the smooth muscle contractile response to PGE2 in various tissues.
Tissue Specificity Abundant in kidney. Lower level expression in lung, skeletal muscle and spleen, lowest expression in testis and not detected in liver brain and heart.
Sequence Similarities Belongs to the G-protein coupled receptor 1 family.
Subcellular Localization Cell membrane; Multi-pass membrane protein.
Uniprot ID P34995
Alternative Names Prostaglandin E2 receptor EP1 subtype;PGE receptor EP1 subtype;PGE2 receptor EP1 subtype;Prostanoid EP1 receptor;PTGER1;
Research Areas |signal transduction|signaling pathway|lipid signaling|prostaglandins| signal transduction|g protein signaling|gpcr|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Prostaglandin E2 receptor EP1 subtype(PGE receptor EP1 subtype/PGE2 receptor EP1 subtype)

PTGER1, Prostaglandin E Receptor 1, comprises 402 amino acids with a predicted molecular mass of 41, 858 and has the 7 predicted transmembrane-spanning domains common to all G protein-coupled receptors. The PTGER1 gene is mapped to chromosome 19p13.1. The protein encoded by this gene is a member of the G protein-coupled receptor family. This protein is one of four receptors identified for prostaglandin E2(PGE2). Through a phosphatidylinositol-calcium second messenger system, Gq proteins mediate this receptor's activity. Knockout studies in mice suggested a role of this receptor in mediating algesia and in regulation of blood pressure. Studies in mice also suggested that this gene may mediate adrenocorticotropic hormone response to bacterial endotoxin.

Anti-Prostaglandin E Receptor EP1 Antibody Images

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Anti-Prostaglandin E Receptor EP1 Antibody
Anti-Prostaglandin E Receptor EP1 antibody, PA1583, Western blotting
All lanes: Anti Prostaglandin E Receptor EP1 (PA1583) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Lane 5: MM453 Whole Cell Lysate at 40ug
Predicted bind size: 42KD
Observed bind size: 42KD
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.