Anti-PSD95/DLG4 Antibody

PSD-95 antibody

Boster Bio Anti-PSD95/DLG4 Antibody catalog # PA2295. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA2295
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, WB

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Product Name

Anti-PSD95/DLG4 Antibody

View all PSD-95 Antibodies

SKU/Catalog Number

PA2295

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PSD95/DLG4 Antibody catalog # PA2295. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PSD95/DLG4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2295)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human PSD95, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2295 is reactive to DLG4 in Human, Mouse, Rat

Applications

PA2295 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

90-100 kDa

Calculated molecular weight

80.495kDa

Background of PSD-95

DLG4 discs large homolog 4, also known as PSD95 or SAP-90, is a protein that in humans is encoded by the DLG4 gene. It is a member of the membrane-associated guanylate kinase (MAGUK) family. This gene is mapped to 17p13.1. DLG4 can heteromultimerize with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Overexpression of DLG4 in hippocampal neurons could drive maturation of glutamatergic synapses. DLG4 can orchestrate synaptic development and it has a role in synapse stabilization and plasticity. Ubiquitination of DLG4 through an MDM2-mediated pathway can regulate AMPA receptor surface expression during synaptic plasticity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat, By Heat
Immunofluorescence, 5 μg/ml, Rat

Validation Images & Assay Conditions

Gene/Protein Information For DLG4 (Source: Uniprot.org, NCBI)

Gene Name

DLG4

Full Name

Disks large homolog 4

Weight

80.495kDa

Superfamily

MAGUK family

Alternative Names

discs, large homolog 4 (Drosophila); disks large homolog 4; DLG4; FLJ97752; FLJ98574; Postsynaptic density protein 95; post-synaptic density protein 95; PSD95; PSD-95; PSD95SAP90SAP-90discs large homolog 4; SAP90; Synapse-associated protein 90; Tax interaction protein 15 DLG4 MRD62, PSD95, SAP-90, SAP90 discs large MAGUK scaffold protein 4 disks large homolog 4|Tax interaction protein 15|discs large homolog 4|post-synaptic density protein 95|synapse-associated protein 90

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on DLG4, check out the DLG4 Infographic

DLG4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DLG4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-PSD95/DLG4 Antibody

Question

Is this PA2295 anti-PSD95/DLG4 antibody reactive to the isotypes of DLG4?

Verified Customer

Verified customer

Asked: 2020-04-13

Answer

The immunogen of PA2295 anti-PSD95/DLG4 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human PSD95(492-507aa RRVERREWSRLKAKDW), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-04-13

Question

I was wanting to use to test anti-PSD95/DLG4 antibody PA2295 on mouse dorsolateral prefrontal cortex for research purposes, then I may be interested in using anti-PSD95/DLG4 antibody PA2295 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-08-02

Answer

The products we sell, including anti-PSD95/DLG4 antibody PA2295, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-08-02

Question

See below the WB image, lot number and protocol we used for dorsolateral prefrontal cortex using anti-PSD95/DLG4 antibody PA2295. Please let me know if you require anything else.

E. Dhar

Verified customer

Asked: 2019-05-10

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-10

Question

Is a blocking peptide available for product anti-PSD95/DLG4 antibody (PA2295)?

J. Mitchell

Verified customer

Asked: 2019-04-19

Answer

We do provide the blocking peptide for product anti-PSD95/DLG4 antibody (PA2295). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-04-19

Question

Can you help my question with product PA2295, anti-PSD95/DLG4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-07-05

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2295 anti-PSD95/DLG4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-07-05

Question

Is there a BSA free version of anti-PSD95/DLG4 antibody PA2295 available?

K. Walker

Verified customer

Asked: 2015-03-24

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-PSD95/DLG4 antibody PA2295 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2015-03-24

Question

Would PA2295 anti-PSD95/DLG4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

H. Li

Verified customer

Asked: 2014-03-03

Answer

You can see on the product datasheet, PA2295 anti-PSD95/DLG4 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-03-03

Order DetailsPrice
PA2295

100μg

$370
PA2295-10ug

10μg sample (liquid)

$99
PA2295-Biotin

100 μg Biotin conjugated

$570
PA2295-Cy3

100 μg Cy3 conjugated

$570
PA2295-Dylight488

100 μg Dylight488 conjugated

$570
PA2295-Dylight550

100 μg Dylight550 conjugated

$570
PA2295-Dylight594

100 μg Dylight594 conjugated

$570
PA2295-FITC

100 μg FITC conjugated

$570
PA2295-HRP

100 μg HRP conjugated

$570
PA2295-APC

100 μg APC conjugated

$670
PA2295-PE

100 μg PE conjugated

$670
PA2295-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PA2295
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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