Anti-PTEN Rabbit Monoclonal Antibody

PTEN antibody

Boster Bio Anti-PTEN Rabbit Monoclonal Antibody catalog # M00006-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: M00006-1
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-PTEN Rabbit Monoclonal Antibody

View all PTEN Antibodies

SKU/Catalog Number

M00006-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-PTEN Rabbit Monoclonal Antibody catalog # M00006-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PTEN Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00006-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

BEI-16

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human PTEN

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00006-1 is reactive to PTEN in Human, Mouse, Rat

Applications

M00006-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

85 kDa

Calculated molecular weight

47.166kDa

Background of PTEN

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The GluR1 subunit is widely expressed throughout the nervous system. Phosphorylation of Ser-845 on GluR1 is thought to be mediated by PKA and phosphorylation of this site increases the conductance of the AMPAR (Roche et al., 1996; Banke et al., 2000). In addition, phosphorylation of this site has been linked to synaptic plasticity as well as learning and memory (Lee at al., 2003; Esteban at al., 2003).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For PTEN (Source: Uniprot.org, NCBI)

Gene Name

PTEN

Full Name

Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN

Weight

47.166kDa

Superfamily

PTEN phosphatase protein family

Alternative Names

BZS; EC 3.1.3.16,10q23del; EC 3.1.3.48; EC 3.1.3.67; GLM2; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; MMAC1; MMAC1MGC11227; Mutated in multiple advanced cancers 1; phosphatase and tensin homologDEC; phosphatidylinositol-34,5-trisphosphate 3-phosphatase and dual-specificityprotein phosphatase PTEN; PTEN; PTEN1; TEP1MHAM PTEN 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC11, PTENbeta, TEP1, PTEN phosphatase and tensin homolog phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN|MMAC1 phosphatase and tensin homolog deleted on chromosome 10|PTENepsilon|mitochondrial PTENalpha|mitochondrial phosphatase and tensin protein alpha|mutated in multiple advanced cancers 1|phosphatase and tensin-like protein|phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN|protein tyrosine phosphatase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PTEN, check out the PTEN Infographic

PTEN infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PTEN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00006-1 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Sun M,Hu L, S,Huang T,Zhang M,Yang M,Zhen W,Yang D,Lu W,Guan M,Peng S. Circulating MicroRNA-19b Identified From Osteoporotic Vertebral Compression Fracture Patients Increases Bone Formation. J Bone Miner Res.2020 Feb;35(2):306-316. doi:10.1002/jbmr.3892.
Species: Human,Mouse
M00006-1 usage in article: APP:WB, SAMPLE:HMSCS CELL AND MC3T3-E1 CELL, DILUTION:1:500

Long-term effects of repeated superovulation on the uterus and mammary gland in rhesus monkeys

Study on the expression and mechanism of inflammatory factors in the brain of rats with cerebral vasospasm

miR-21 Reduces Hydrogen Peroxide-Induced Apoptosis in c-kit

miR-21 increases c-kit cardiac stem cell proliferation in vitro through PTEN/PI3K/Akt signaling

Expression and significance of PTEN, hypoxia-inducible factor-1 alpha in colorectal adenoma and adenocarcinoma

Prognostic role of PPAR-%u03B3 and PTEN in the renal cell carcinoma

Pin1 Promotes Regulated Necrosis Induced by Glutamate in Rat Retinal Neurons via CAST/Calpain2 Pathway

708: Combined Analysis of EGFR and PTEN Status in Patients With KRAS Wild-Type Metastatic Colorectal Cancer

Overexpression of LRIG1 regulates PTEN via MAPK/MEK signaling pathway in esophageal squamous cell carcinoma

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16 Customer Q&As for Anti-PTEN Rabbit Monoclonal Antibody

Question

We are currently using anti-PTEN Rabbit Monoclonal antibody M00006-1 for rat tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-26

Answer

The anti-PTEN Rabbit Monoclonal antibody (M00006-1) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-26

Question

Would M00006-1 anti-PTEN Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-02-20

Answer

It shows on the product datasheet, M00006-1 anti-PTEN Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-02-20

Question

Is a blocking peptide available for product anti-PTEN Rabbit Monoclonal antibody (M00006-1)?

Verified Customer

Verified customer

Asked: 2019-12-19

Answer

We do provide the blocking peptide for product anti-PTEN Rabbit Monoclonal antibody (M00006-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-12-19

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for epithelium using anti-PTEN Rabbit Monoclonal antibody M00006-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-11-29

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-29

Question

Our lab want to know about to test anti-PTEN Rabbit Monoclonal antibody M00006-1 on mouse epithelium for research purposes, then I may be interested in using anti-PTEN Rabbit Monoclonal antibody M00006-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-09-02

Answer

The products we sell, including anti-PTEN Rabbit Monoclonal antibody M00006-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-09-02

Question

I was wanting to use your anti-PTEN Rabbit Monoclonal antibody for IF for mouse epithelium on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse epithelium identification?

Verified Customer

Verified customer

Asked: 2019-08-15

Answer

As indicated on the product datasheet, M00006-1 anti-PTEN Rabbit Monoclonal antibody has been tested for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse epithelium in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-15

Question

Would anti-PTEN Rabbit Monoclonal antibody M00006-1 work on goat IF with spleen?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

Our lab technicians have not validated anti-PTEN Rabbit Monoclonal antibody M00006-1 on goat. You can run a BLAST between goat and the immunogen sequence of anti-PTEN Rabbit Monoclonal antibody M00006-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat spleen in IF, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-24

Question

Please see the WB image, lot number and protocol we used for epithelium using anti-PTEN Rabbit Monoclonal antibody M00006-1. Please let me know if you require anything else.

C. Johnson

Verified customer

Asked: 2018-12-14

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-12-14

Question

We have been able to see staining in mouse intestine. What should we do? Is anti-PTEN Rabbit Monoclonal antibody supposed to stain intestine positively?

W. Krishna

Verified customer

Asked: 2018-03-19

Answer

According to literature intestine does express PTEN. According to Uniprot.org, PTEN is expressed in intestine, epithelium, spleen, lung, liver, among other tissues. Regarding which tissues have PTEN expression, here are a few articles citing expression in various tissues:
Epithelium, Pubmed ID: 9187108, 9090379
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 15489334
Spleen, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2018-03-19

Question

I see that the anti-PTEN Rabbit Monoclonal antibody M00006-1 works with IF, what is the protocol used to produce the result images on the product page?

K. Anderson

Verified customer

Asked: 2018-02-08

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-02-08

Question

We ordered your anti-PTEN Rabbit Monoclonal antibody for IP on spleen last year. I am using rat, and I plan to use the antibody for WB next. Our lab want to know about examining spleen as well as epithelium in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

C. Huang

Verified customer

Asked: 2018-02-05

Answer

I took a look at the website and datasheets of our anti-PTEN Rabbit Monoclonal antibody and it seems that M00006-1 has been validated on rat in both IP and WB. Thus M00006-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-02-05

Question

Do you have a BSA free version of anti-PTEN Rabbit Monoclonal antibody M00006-1 available?

Verified Customer

Verified customer

Asked: 2018-02-01

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PTEN Rabbit Monoclonal antibody M00006-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-02-01

Question

Is this M00006-1 anti-PTEN Rabbit Monoclonal antibody reactive to the isotypes of PTEN?

Verified Customer

Verified customer

Asked: 2018-01-09

Answer

The immunogen of M00006-1 anti-PTEN Rabbit Monoclonal antibody is A synthesized peptide derived from human PTEN . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-01-09

Question

We were content with the WB result of your anti-PTEN Rabbit Monoclonal antibody. However we have been able to see positive staining in epithelium cytoplasm using this antibody. Is that expected? Could you tell me where is PTEN supposed to be expressed?

K. Rodriguez

Verified customer

Asked: 2015-04-23

Answer

From literature, epithelium does express PTEN. Generally PTEN expresses in cytoplasm. Regarding which tissues have PTEN expression, here are a few articles citing expression in various tissues:
Epithelium, Pubmed ID: 9187108, 9090379
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 15489334
Spleen, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2015-04-23

Question

My question regarding product M00006-1, anti-PTEN Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

B. Edwards

Verified customer

Asked: 2014-05-28

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00006-1 anti-PTEN Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-05-28

Question

Will anti-PTEN Rabbit Monoclonal antibody M00006-1 work for IF with epithelium?

T. Krishna

Verified customer

Asked: 2013-05-24

Answer

According to the expression profile of epithelium, PTEN is highly expressed in epithelium. So, it is likely that anti-PTEN Rabbit Monoclonal antibody M00006-1 will work for IF with epithelium.

Boster Scientific Support

Answered: 2013-05-24

Order DetailsPrice
M00006-1

100μL

$370
M00006-1-30ul

30μL sample

$99
M00006-1-carrier-free

Carrier Free

$370

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