|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-RAB13 Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Ras-related protein Rab-13(RAB13) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-RAB13 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9790)|
|Replacement Item||This antibody may replace the following items: sc-56182|sc-99|sc-98|sc-53396|sc-53397|sc-1314|sc-1312|sc-65226|sc-6243|sc-374087|sc-47698|sc-53394|sc-17846|sc-55476|sc-263|sc-73566|sc-81168|sc-56180|sc-65334|sc-136023|sc-71821|sc-71820|sc-71818|sc-71819|sc-71817|sc-17577|sc-17578|sc-25767|sc-1313|sc-100|sc-74573|sc-1315|sc-74574|sc-126|sc-393031|sc-6243-G|sc-56179|sc-71815|sc-98-X|sc-6243-X|sc-126-X|sc-1311-R|sc-71817-X|sc-1315-X from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human RAB13 (121-150aa NKCDMEAKRKVQKEQADKLAREHGIRFFET), different from the related mouse and rat sequences by four amino acids.|
|Cross Reactivity||No cross reactivity with other proteins.|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Ras-related protein Rab-13|
|Molecular Weight||22774 MW|
|Protein Function||The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in endocytic recycling and regulates the transport to the plasma membrane of transmembrane proteins like the tight junction protein OCLN/occludin. Thereby, it regulates the assembly and the activity of tight junctions. Moreover, it may also regulate tight junction assembly by activating the PKA signaling pathway and by reorganizing the actin cytoskeleton through the activation of the downstream effectors PRKACA and MICALL2 respectively. Through its role in tight junction assembly, may play a role in the establishment of Sertoli cell barrier. Plays also a role in angiogenesis through regulation of endothelial cells chemotaxis. Also involved in neurite outgrowth. Has also been proposed to play a role in post-Golgi membrane trafficking from the TGN to the recycling endosome. Finally, it has been involved in insulin- induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore may play a role in glucose homeostasis. .|
|Tissue Specificity||Detected in several types of epithelia, including intestine, kidney, liver and in endothelial cells.|
|Subcellular Localization||Cell membrane ; Lipid-anchor ; Cytoplasmic side . Cytoplasmic vesicle membrane ; Lipid- anchor ; Cytoplasmic side . Cell junction, tight junction . Golgi apparatus, trans-Golgi network membrane . Recycling endosome membrane . Cell projection, lamellipodium . Tight junctions or associated with vesicles scattered throughout the cytoplasm in cells lacking tight junctions (PubMed:8294494). Relocalizes to the leading edge of lamellipodia in migrating endothelial cells (By similarity). .|
|Alternative Names||Ras-related protein Rab-13;Cell growth-inhibiting gene 4 protein;RAB13;GIG4;|
|Research Areas|||signal transduction|signaling pathway|g protein signaling|small g proteins|ras family| signal transduction|cytoskeleton / ecm|cell adhesion|tight junctions||
Background for Ras-related protein Rab-13
Anti-RAB13 Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti RAB13 (PB9790) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 23KD, 45KD
Observed bind size: 23KD, 45KD
IHC(P): Mouse Intestine Tissue
IHC(P): Rat Intestine Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,