Anti-Phospho-Raf-1 (Ser642) Antibody

Raf-1 antibody

Boster Bio Anti-Phospho-Raf-1 (Ser642) Antibody (Catalog # P00446-1). Tested in WB applications. This antibody reacts with Human, Rat.

Product Info Summary

SKU: P00446-1
Size: 100 µl
Reactive Species: Human, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-Phospho-Raf-1 (Ser642) Antibody

View all Raf-1 Antibodies

SKU/Catalog Number

P00446-1

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-Raf-1 (Ser642) Antibody (Catalog # P00446-1). Tested in WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-Raf-1 (Ser642) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00446-1)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser642 of rat Raf-1, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P00446-1 is reactive to RAF1 in Human, Rat

Applications

P00446-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

73.052kDa

Background of Raf-1

The Ras pathway is a critical signal transduction cascade involved in regulating cellular proliferation, differentiation, survival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases MEK and ERK (Marshall, 1996). Previous studies have shown that phosphorylation is required for Raf-1 activation (Dhillon and Kolch, 2002; Chong et al., 2003). Recent work has demonstrated that phosphorylation also regulates the downregulation of Raf (Dougherty et al., 2005) with two sites participating: Ser-301 and Ser-642.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

Gene/Protein Information For RAF1 (Source: Uniprot.org, NCBI)

Gene Name

RAF1

Full Name

RAF proto-oncogene serine/threonine-protein kinase

Weight

73.052kDa

Superfamily

protein kinase superfamily

Alternative Names

cRaf; c-Raf; c-Raf-1; CRAFraf proto-oncogene serine/threonine protein kinase; EC 2.7.11; EC 2.7.11.1; NS5; Proto-oncogene c-RAF; RAF proto-oncogene serine/threonine-protein kinase; RAF; Raf1; Raf-1; Raf-1Oncogene RAF1; v-raf-1 murine leukemia viral oncogene homolog 1; v-Raf-1 RAF1 CMD1NN, CRAF, NS5, Raf-1, c-Raf Raf-1 proto-oncogene, serine/threonine kinase RAF proto-oncogene serine/threonine-protein kinase|C-Raf proto-oncogene, serine/threonine kinase|Oncogene RAF1|proto-oncogene c-RAF|raf proto-oncogene serine/threonine protein kinase|v-raf-1 murine leukemia viral oncogene homolog 1|v-raf-1 murine leukemia viral oncogene-like protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on RAF1, check out the RAF1 Infographic

RAF1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RAF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
P00446-1

100uL

$465

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P00446-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$465.00

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