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SKU:PA1069
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC-P, IHC-F, WB
Price: $200.00
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Overview

Product Name Anti-RAGE Antibody
SKU/Catalog Number PA1069
Description Rabbit IgG polyclonal antibody for Advanced glycosylation end product-specific receptor(AGER) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.
Cite This Product Anti-RAGE Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1069)
Replacement Item This antibody may replace the following items: sc-33662|sc-365154|sc-5563|sc-74473|sc-74535|sc-80652|sc-80653|sc-8229|sc-8230 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, IHC-F, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human RAGE(174-192aa KEQTRRHPETGLFTLQSEL), different from the related mouse and rat sequences by two amino acids.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name AGER
Protein Name Advanced glycosylation end product-specific receptor
Molecular Weight 42803 MW
Protein Function Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF- alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Can also bind oligonucleotides. .
Tissue Specificity Endothelial cells.
Sequence Similarities Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Subcellular Localization Isoform 1: Cell membrane; Single-pass type I membrane protein.
Uniprot ID Q15109
Alternative Names Advanced glycosylation end product-specific receptor;Receptor for advanced glycosylation end products;AGER;RAGE;
Research Areas |neuroscience|neurology process|neurodegenerative disease|alzheimer's disease|amyloid| neuroscience|sensory system|visual system| cardiovascular|atherosclerosis|diabetes associated|vascular inflammation|inflammatory mediators|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Advanced glycosylation end product-specific receptor

The receptor for advanced glycation end products(RAGE) is a multi-ligand member of the immunoglobulin superfamily of cell surface molecules. It interacts with distinct molecules implicated in homeostasis, development and inflammation, and certain diseases such as diabetes and Alzheimer's disease. RAGE is also a central cell surface receptor for amphoterin and EN-RAGE. RAGE is associated with sustained NF-kappaB activation in the diabetic microenvironment and has a central role in sensory neuronal dysfunction. Moreover, RAGE propagates cellular dysfunction in several inflammatory disorders and diabetes, and it also functions as an endothelial adhesion receptor promoting leukocyte recruitment.

Anti-RAGE Antibody Images

Click the images to enlarge.

Anti-RAGE Antibody
Anti-RAGE antibody, PA1069, Western blotting
Lane 1: Recombinant Human RAGE Protein 10ng
Lane 2: Recombinant Human RAGE Protein 5ng
Lane 3: Recombinant Human RAGE Protein 2.5ng
Anti-RAGE Antibody
Anti-RAGE antibody, PA1069, IHC(P)
IHC(P): Rat Lung Tissue
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Publications

Qin Q, Niu J, Wang Z, Xu W, Qiao Z, Gu Y. Cardiovasc Diabetol. 2013 Feb 26;12:37. Doi: 10.1186/1475-2840-12-37. Heparanase Induced By Advanced Glycation End Products (Ages) Promotes Macrophage Migration Involving Rage And Pi3K/Akt Pathway.
Liu J, Wang S, Feng L, Ma D, Fu Q, Song Y, Jia X, Ma S. J Med Food. 2013 Jul;16(7):577-86. Doi: 10.1089/Jmf.2012.2654. Hypoglycemic And Antioxidant Activities Of Paeonol And Its Beneficial Effect On Diabetic Encephalopathy In Streptozotocin-Induce...
Wang L, Zhang X, Liu L, Yang R, Cui L, Li M. Neurosci Lett. 2010 Mar 8;471(3):152-6. Doi: 10.1016/J.Neulet.2010.01.030. Epub 2010 Jan 25. Atorvastatin Protects Rat Brains Against Permanent Focal Ischemia And Downregulates Hmgb1, Hmgb1 Receptors (R...
Wang L, Zhang X, Liu L, Cui L, Yang R, Li M, Du W. Brain Res. 2010 Mar 19;1321:143-51. Doi: 10.1016/J.Brainres.2009.12.046. Epub 2010 Jan 4. Tanshinone Ii A Down-Regulates Hmgb1, Rage, Tlr4, Nf-Kappab Expression, Ameliorates Bbb Permeability And E...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.