Anti-Ran Antibody Picoband™ (monoclonal, 5D5)

RAN antibody

Boster Bio Anti-Ran Antibody Picoband™ (monoclonal, 5D5) catalog # M00204-1. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M00204-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: Flow Cytometry, IF, ICC, WB

Customers Who Bought This Also Bought

Product Name

Anti-Ran Antibody Picoband™ (monoclonal, 5D5)

View all RAN Antibodies

SKU/Catalog Number

M00204-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Ran Antibody Picoband™ (monoclonal, 5D5) catalog # M00204-1. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Ran Antibody Picoband™ (monoclonal, 5D5) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00204-1)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

5D5

Isotype

Mouse IgG2b

Immunogen

E. coli-derived human Ran recombinant protein (Position: A2-L216). Human Ran shares 100% amino acid (aa) sequence identity with both mouse and rat Ran.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M00204-1 is reactive to RAN in Human, Mouse, Rat

Applications

M00204-1 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

24 kDa

Calculated molecular weight

24.423kDa

Background of RAN

RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For RAN (Source: Uniprot.org, NCBI)

Gene Name

RAN

Full Name

GTP-binding nuclear protein Ran

Weight

24.423kDa

Superfamily

small GTPase superfamily

Alternative Names

Androgen receptor-associated protein 24; ARA24Gsp1; GTPase Ran; GTP-binding nuclear protein Ran; guanosine triphosphatase Ran; member RAS oncogene family; OK/SW-cl.81; RAN, member RAS oncogene family; RanGTPase; Ras-like protein TC4; Ras-related nuclear protein; TC4 RAN ARA24, Gsp1, TC4 RAN, member RAS oncogene family GTP-binding nuclear protein Ran|GTPase Ran|RanGTPase|androgen receptor-associated protein 24|guanosine triphosphatase Ran|member RAS oncogene family|ras-like protein TC4|ras-related nuclear protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on RAN, check out the RAN Infographic

RAN infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RAN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for M00204-1

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-Ran Antibody Picoband™ (monoclonal, 5D5)?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Ran Antibody Picoband™ (monoclonal, 5D5)

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

6 Customer Q&As for Anti-Ran Antibody Picoband™ (monoclonal, 5D5)

Question

you antibody using your anti-Ran antibody (monoclonal, 5D5) for pre-mirna export from nucleus studies. Has this antibody been tested with western blotting on mouse lung tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-08-07

Answer

Thank you for your inquiry. This M00204-1 anti-Ran antibody (monoclonal, 5D5) is tested on human a549, a549 whole cell lysates, u2os whole cell lysates, hepg2 whole cell lysates, sw620 whole cell lysates, mouse lung tissue, rat testis tissue, kidney tissue. It is guaranteed to work for Flow Cytometry, IF, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-08-07

Question

Do you have related data for WB, IF and IHC used by M00204-1?

Verified customer

Asked: 2019-04-23

Answer

For the Anti-Ran Antibody Picoband™ (Monoclonal, 5D5) (M00204-1), Please see the images on the product page. https://www.bosterbio.com/anti-ran-picoband-trade-antibody-monoclonal-m00204-1-boster.html

Boster Scientific Support

Answered: 2019-04-23

Question

We purchased anti-Ran antibody (monoclonal, 5D5) for Flow Cytometry on lymphoblast a few months ago. I am using rat, and We are going to use the antibody for ICC next. We are interested in examining lymphoblast as well as umbilical cord blood in our next experiment. Do you have any suggestion on which antibody would work the best for ICC?

Verified Customer

Verified customer

Asked: 2018-10-03

Answer

I took a look at the website and datasheets of our anti-Ran antibody (monoclonal, 5D5) and I see that M00204-1 has been validated on rat in both Flow Cytometry and ICC. Thus M00204-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-10-03

Question

We are currently using anti-Ran antibody (monoclonal, 5D5) M00204-1 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?

G. Huang

Verified customer

Asked: 2014-08-29

Answer

The anti-Ran antibody (monoclonal, 5D5) (M00204-1) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-08-29

Question

We have been able to see staining in human umbilical cord blood. Do you have any suggestions? Is anti-Ran antibody (monoclonal, 5D5) supposed to stain umbilical cord blood positively?

D. Roberts

Verified customer

Asked: 2014-02-24

Answer

From what I have seen in literature umbilical cord blood does express RAN. From what I have seen in Uniprot.org, RAN is expressed in testis, teratocarcinoma, brain, umbilical cord blood, hippocampus, lymph, ovary, skin uterus, platelet, brain cajal-retzius cell, colon adenocarcinoma, cervix carcinoma, lymphoblast, melanoma, erythroleukemia, liver, among other tissues. Regarding which tissues have RAN expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8421051, 10400640
Cervix carcinoma, Pubmed ID: 1961752
Erythroleukemia, Pubmed ID: 23186163
Hippocampus, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Lymph, Ovary, Skin, and Uterus, Pubmed ID: 15489334
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065
Teratocarcinoma, Pubmed ID: 2108320
Umbilical cord blood, Pubmed ID: 11042152

Boster Scientific Support

Answered: 2014-02-24

Question

Our lab were satisfied with the WB result of your anti-Ran antibody (monoclonal, 5D5). However we have observed positive staining in platelet nucleus using this antibody. Is that expected? Could you tell me where is RAN supposed to be expressed?

C. Lewis

Verified customer

Asked: 2014-02-20

Answer

From literature, platelet does express RAN. Generally RAN expresses in nucleus. Regarding which tissues have RAN expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8421051, 10400640
Cervix carcinoma, Pubmed ID: 1961752
Erythroleukemia, Pubmed ID: 23186163
Hippocampus, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Lymph, Ovary, Skin, and Uterus, Pubmed ID: 15489334
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065
Teratocarcinoma, Pubmed ID: 2108320
Umbilical cord blood, Pubmed ID: 11042152

Boster Scientific Support

Answered: 2014-02-20

Order DetailsPrice
M00204-1

100μg

$370
M00204-1-10ug

10μg sample (liquid)

$99
M00204-1-Biotin

100 μg Biotin conjugated

$570
M00204-1-Cy3

100 μg Cy3 conjugated

$570
M00204-1-Dylight488

100 μg Dylight488 conjugated

$570
M00204-1-Dylight550

100 μg Dylight550 conjugated

$570
M00204-1-Dylight594

100 μg Dylight594 conjugated

$570
M00204-1-FITC

100 μg FITC conjugated

$570
M00204-1-HRP

100 μg HRP conjugated

$570
M00204-1-APC

100 μg APC conjugated

$670
M00204-1-PE

100 μg PE conjugated

$670
M00204-1-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
M00204-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.