Anti-Raptor RPTOR Rabbit Monoclonal Antibody

RPTOR antibody

Boster Bio Anti-Raptor RPTOR Rabbit Monoclonal Antibody catalog # M01463. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M01463
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

Product Name

Anti-Raptor RPTOR Rabbit Monoclonal Antibody

View all RPTOR Antibodies

SKU/Catalog Number

M01463

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Raptor RPTOR Rabbit Monoclonal Antibody catalog # M01463. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Raptor RPTOR Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01463)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

EGB-18

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Raptor

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M01463 is reactive to RPTOR in Human, Mouse, Rat

Applications

M01463 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

43 kDa

Calculated molecular weight

149.038kDa

Background of RPTOR

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:1000
IHC 1:50-1:100
ICC/IF 1:50-1:100
IP 1:30
FC 1:30

Validation Images & Assay Conditions

Gene/Protein Information For RPTOR (Source: Uniprot.org, NCBI)

Gene Name

RPTOR

Full Name

Regulatory-associated protein of mTOR

Weight

149.038kDa

Superfamily

WD repeat RAPTOR family

Alternative Names

KIAA1303; KIAA1303p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats; KOG1; Mip1; p150 target of rapamycin (TOR)-scaffold protein; Raptor; regulatory associated protein of MTOR, complex 1; regulatory-associated protein of mTOR; RPTOR RPTOR KOG1, Mip1 regulatory associated protein of MTOR complex 1 regulatory-associated protein of mTOR|p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats|raptor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on RPTOR, check out the RPTOR Infographic

RPTOR infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RPTOR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-Raptor RPTOR Rabbit Monoclonal Antibody

Question

Is a blocking peptide available for product anti-Raptor Rabbit Monoclonal antibody (M01463)?

Verified Customer

Verified customer

Asked: 2019-03-05

Answer

We do provide the blocking peptide for product anti-Raptor Rabbit Monoclonal antibody (M01463). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-03-05

Question

Does anti-Raptor Rabbit Monoclonal antibody M01463 work on horse IP with brain?

Verified Customer

Verified customer

Asked: 2018-12-14

Answer

Our lab technicians have not tested anti-Raptor Rabbit Monoclonal antibody M01463 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Raptor Rabbit Monoclonal antibody M01463 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse brain in IP, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-12-14

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-Raptor Rabbit Monoclonal antibody M01463. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-09-24

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-09-24

Question

Would anti-Raptor Rabbit Monoclonal antibody M01463 work for IP with leukemic t-cell?

Verified Customer

Verified customer

Asked: 2017-11-07

Answer

According to the expression profile of leukemic t-cell, RPTOR is highly expressed in leukemic t-cell. So, it is likely that anti-Raptor Rabbit Monoclonal antibody M01463 will work for IP with leukemic t-cell.

Boster Scientific Support

Answered: 2017-11-07

Question

We are currently using anti-Raptor Rabbit Monoclonal antibody M01463 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?

K. Mangal

Verified customer

Asked: 2016-12-14

Answer

The anti-Raptor Rabbit Monoclonal antibody (M01463) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-12-14

Order DetailsPrice
M01463

100μL

$370
M01463-30ul

30μL sample

$99
M01463-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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