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Product Info Summary
| SKU: | A00392 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC-P, WB |
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Product info
Product Name
Anti-RARA Antibody (C-term)
View all RAR alpha/NR1B1 Antibodies
SKU/Catalog Number
A00392
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-RARA Antibody (C-term) (Catalog # A00392). Tested in WB, Flow Cytometry, IHC-P, IF application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-RARA Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00392)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This RARA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-349 amino acids from the C-terminal region of human RARA.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00392 is reactive to RARA in Human, Mouse
Reconstitution
Calculated molecular weight
50771 Da
Background of RAR alpha/NR1B1
This gene represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00392 is guaranteed for Flow Cytometry, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:10-1:50
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
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Confocal immunofluorescent analysis of RARA Antibody (C-term) with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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All lanes : Anti-RARA Antibody (C-term) at 1:1000 dilution
Lane 1: HL-60 whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: Hela whole cell lysate
Lane 5: T47D whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 51 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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RARA Antibody (C-term) (Cat. #A00392) western blot analysis in HL-60 cell line and mouse testis tissue lysates (35ug/lane). This demonstrates the RARA antibody detected the RARA protein (arrow).
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RARA Antibody (C-term) (Cat. #A00392)immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of RARA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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RARA Antibody (C-term) (Cat. #A00392) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For RARA (Source: Uniprot.org, NCBI)
Gene Name
RARA
Full Name
Retinoic acid receptor alpha
Weight
50771 Da
Superfamily
nuclear hormone receptor family
Alternative Names
Retinoic acid receptor alpha, RAR-alpha, Nuclear receptor subfamily 1 group B member 1, RARA, NR1B1 RARA NR1B1, RAR retinoic acid receptor alpha retinoic acid receptor alpha|RAR-alpha|nuclear receptor subfamily 1 group B member 1|nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form|retinoic acid nuclear receptor alpha variant 1|retinoic acid nuclear receptor alpha variant 2|retinoic acid receptor, alpha polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RARA, check out the RARA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RARA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RARA Antibody (C-term) (A00392)
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